Voss Oliver H, Lee Ha-Na, Tian Linjie, Krzewski Konrad, Coligan John E
Receptor Cell Biology Section, Laboratory of Immunogenetics, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland.
Curr Protoc Immunol. 2018 Feb 21;120:14.44.1-14.44.21. doi: 10.1002/cpim.43.
Efficient phagocytosis of apoptotic cells (efferocytosis) is essential for immune homeostasis. Phospholipids exposed on the surface of apoptotic cells, such as phosphatidylserine, supply important "eat-me" signals. Liposomes are lipid bilayer vesicles that can be generated from one or several types of phospholipids of interest. Thus, these vesicles offer versatility, flexibility, and, importantly, a three-dimensional structure for studying the interaction between lipids and their receptors as well as the lipid-receptor interaction-mediated signaling events controlling efferocytosis by cells like professional phagocytes. Here, we describe methods to prepare liposomes, perform liposome-based lipid-receptor binding assays, use liposomes to block efferocytosis, and utilize liposome-coated beads as apoptotic cell surrogates for phagocytosis. © 2018 by John Wiley & Sons, Inc.
凋亡细胞的高效吞噬作用（胞葬作用）对于免疫稳态至关重要。凋亡细胞表面暴露的磷脂，如磷脂酰丝氨酸，提供重要的“吃我”信号。脂质体是脂质双层囊泡，可由一种或几种感兴趣的磷脂生成。因此，这些囊泡具有多功能性、灵活性，重要的是，具有三维结构，可用于研究脂质与其受体之间的相互作用以及脂质-受体相互作用介导的信号事件，这些信号事件控制着专业吞噬细胞等细胞的胞葬作用。在这里，我们描述了制备脂质体、进行基于脂质体的脂质-受体结合测定、使用脂质体阻断胞葬作用以及利用脂质体包被的珠子作为凋亡细胞替代物进行吞噬作用的方法。© 2018约翰威立父子公司
