Marschall H U, Egestad B, Matern H, Matern S, Sjövall J
FEBS Lett. 1987 Mar 23;213(2):411-4. doi: 10.1016/0014-5793(87)81532-6.
A glucosyltransferase catalysing formation of bile acid glucosides was recently isolated from human liver microsomes. In order to investigate the potential occurrence of such bile acid derivatives in vivo, a method was devised for their isolation and purification from urine. Conditions were established with the aid of glucosides of radiolabelled, unconjugated glycine and taurine conjugated bile acids prepared enzymatically using human liver microsomes. Analysis by gas chromatography and mass spectrometry of methyl ester trimethylsilyl ether derivatives indicated the excretion of glucosides of nonamidated hyodeoxycholic, chenodeoxycholic, deoxycholic, ursodeoxycholic and cholic acids and of glycine and taurine conjugated chenodeoxycholic and cholic acids. Additional compounds were present giving mass spectral fragmentation patterns typical of di- and trihydroxy bile acid glycosides. Semiquantitative estimates indicated a total daily excretion of about 1 mumol.
最近从人肝微粒体中分离出一种催化胆汁酸葡萄糖苷形成的葡萄糖基转移酶。为了研究此类胆汁酸衍生物在体内的潜在存在情况，设计了一种从尿液中分离和纯化它们的方法。借助使用人肝微粒体酶促制备的放射性标记的未结合甘氨酸和牛磺酸结合胆汁酸的葡萄糖苷来确定条件。对甲酯三甲基硅醚衍生物进行气相色谱和质谱分析表明，未酰胺化的猪去氧胆酸、鹅去氧胆酸、脱氧胆酸、熊去氧胆酸和胆酸以及甘氨酸和牛磺酸结合的鹅去氧胆酸和胆酸的葡萄糖苷会排出。还存在其他化合物，其质谱裂解模式为二羟基和三羟基胆汁酸糖苷所特有。半定量估计表明每日总排泄量约为1微摩尔。
