人肝脏、肾脏和肠道中的微粒体葡萄糖基转移酶形成胆汁酸葡萄糖苷和多萜醇磷酸葡萄糖。

Formation of bile acid glucosides and dolichyl phosphoglucose by microsomal glucosyltransferases in liver, kidney and intestine of man.

作者信息

Matern H, Matern S

出版信息

Biochim Biophys Acta. 1987 Sep 4;921(1):1-6. doi: 10.1016/0005-2760(87)90163-9.

Abstract

Formation of glucosides of the bile acids chenodeoxycholic, ursodeoxycholic, deoxycholic and hyodeoxycholic acids has been detected in microsomes from human liver, kidney and intestinal mucosa. Hepatic and extrahepatic bile acid glucosyltransferase activities were characterized with respect to kinetic parameters and other catalytic properties. Whereas no marked organ-specific differences in the affinities of individual bile acids toward hepatic and extrahepatic glucosyltransferases were observed, microsomes from extrahepatic sources showed twice to 5-times the maximal rates of bile acid glucosidation estimated with microsomes from liver. In addition to bile acid glucoside formation, microsomes from human liver, kidney and intestinal mucosa catalyzed the synthesis of dolichyl phosphoglucose acting as natural glucosyl donor in bile acid glucosidation.

摘要

在人肝脏、肾脏和肠黏膜的微粒体中已检测到鹅去氧胆酸、熊去氧胆酸、脱氧胆酸和猪去氧胆酸的胆汁酸葡糖苷的形成。对肝脏和肝外胆汁酸葡糖基转移酶活性的动力学参数及其他催化特性进行了表征。尽管未观察到各胆汁酸对肝脏和肝外葡糖基转移酶亲和力有明显的器官特异性差异，但肝外来源的微粒体显示出的胆汁酸葡糖基化最大速率是肝脏微粒体估计值的2至5倍。除了胆汁酸葡糖苷的形成外，人肝脏、肾脏和肠黏膜的微粒体还催化了多萜醇磷酸葡萄糖的合成，其在胆汁酸葡糖基化中作为天然葡糖基供体。