Ito N, Noguchi K, Kazama M, Shimura K, Kasai K
J Chromatogr. 1987 Jan 16;386:51-6. doi: 10.1016/s0021-9673(01)94583-2.
A procedure for the analysis of the fibrinolytic system in human blood was devised by combining high-performance affinity chromatography (HPAC) and specific detection of proenzyme. Components of the fibrinolytic system were separated by HPAC using Asahipak GS gel coupled with p-aminobenzamidine, and they were specifically detected by means of an on-line enzyme assay system. This system made it possible to quantitate not only Glu-plasminogen (Glu-Plg) but also Lys-plasminogen (Lys-Plg) in human plasma in a short time without pre-treatment. The effect of urokinase on the state of components of the fibrinolytic system in blood was studied. It was clearly shown that Lys-Plg is more susceptible to activation by urokinase than Glu-Plg (both in vitro and in vivo).
通过结合高效亲和色谱法（HPAC）和酶原的特异性检测，设计了一种分析人体血液中纤维蛋白溶解系统的方法。使用与对氨基苯甲脒偶联的Asahipak GS凝胶，通过HPAC分离纤维蛋白溶解系统的成分，并通过在线酶分析系统对其进行特异性检测。该系统能够在无需预处理的情况下，在短时间内对人体血浆中的谷氨酸纤溶酶原（Glu-Plg）和赖氨酸纤溶酶原（Lys-Plg）进行定量分析。研究了尿激酶对血液中纤维蛋白溶解系统成分状态的影响。结果清楚地表明，无论是在体外还是体内，Lys-Plg比Glu-Plg更容易被尿激酶激活。
