Ito N, Abe I, Noguchi K, Kazama M, Shimura K, Kasai K
Gel Separation Development Department, Asahi Chemical Industry Co., Ltd., Kawasaki, Japan.
Biochem Int. 1987 Aug;15(2):311-9.
Human plasminogens specifically separated by high-performance affinity chromatography were specifically detected by a newly devised, on-line monitoring system, in which the proenzymes were activated by urokinase and plasmin activity thus generated was assayed. Presence of Lys-plasminogen as a constituent in the blood was demonstrated by both chromatographic patterns and biochemical experiments. This system made it possible to estimate rapidly not only Glu-plasminogen but also Lys-plasminogen in the plasma without any pretreatment. Its utility as a tool for clinical analysis of fibrinolytic system was suggested.
通过高效亲和色谱法特异性分离得到的人纤溶酶原，采用新设计的在线监测系统进行特异性检测，该系统中通过尿激酶激活酶原，并测定由此产生的纤溶酶活性。通过色谱图谱和生化实验均证实血液中存在赖氨酸纤溶酶原这一成分。该系统不仅可以在无需任何预处理的情况下快速估算血浆中的谷氨酸纤溶酶原，还能估算赖氨酸纤溶酶原。提示了其作为纤溶系统临床分析工具的实用性。
