Abrão Fariza, Alves Jessica A, Andrade Gessica, de Oliveira Pollyanna F, Ambrósio Sérgio R, Veneziani Rodrigo C S, Tavares Denise C, Bastos Jairo K, Martins Carlos H G
Research Laboratory of Applied Microbiology, University of Franca, São Paulo, Brazil.
Laboratory of Mutagenesis, University of Franca, São Paulo, Brazil.
Front Microbiol. 2018 Feb 21;9:201. doi: 10.3389/fmicb.2018.00201. eCollection 2018.
This study evaluates the antibacterial activity of the Dwyer oleoresin and two isolated compounds [eperu-8(20)-15,18-dioic acid and polyalthic acid] against bacteria involved in primary endodontic infections and dental caries and assesses the cytotoxic effect of these substances against a normal cell line. MIC and MBC assays pointed out the most promising metabolites for further studies on bactericidal kinetics, antibiofilm activity, and synergistic antibacterial action. The oleoresin and polyalthic acid but not eperu-8(20)-15,18-dioic provided encouraging MIC and MBC results at concentrations lower than 100 μg mL. The oleoresin and polyalthic acid activities depended on the evaluated strain. A bactericidal effect on (ATCC 11578 and clinical isolate) emerged before 8 h of incubation. For all the tested bacteria, the oleoresin and polyalthic acid inhibited biofilm formation by at least 50%. The oleoresin and polyalthic acid gave the best activity against (ATCC 19039) and (ATCC 11578), respectively. The synergistic assays combining the oleoresin or polyalthic acid with chlorhexidine did not afford interesting results. We examined the cytotoxicity of oleoresin, eperu-8(20)-15,18-dioic acid, and polyalthic acid against Chinese hamster lung fibroblasts. The oleoresin and polyalthic acid were cytotoxic at concentrations above 78.1 μg mL, whereas eperu-8(20)-15,18-dioic displayed cytotoxicity at concentrations above 312.5 μg mL. In conclusion, the oleoresin and polyalthic acid are potential sources of antibacterial agents against bacteria involved in primary endodontic infections and dental caries in both the sessile and the planktonic modes at concentrations that do not cause cytotoxicity.
本研究评估了德怀尔油树脂及两种分离化合物[埃佩鲁 - 8(20)-15,18 - 二酸和多花含笑酸]对引起原发性牙髓感染和龋齿的细菌的抗菌活性，并评估了这些物质对正常细胞系的细胞毒性作用。最低抑菌浓度（MIC）和最低杀菌浓度（MBC）测定指出了最有前景的代谢物，可用于进一步研究杀菌动力学、抗生物膜活性和协同抗菌作用。油树脂和多花含笑酸在浓度低于100μg/mL时提供了令人鼓舞的MIC和MBC结果，但埃佩鲁 - 8(20)-15,18 - 二酸未达到此效果。油树脂和多花含笑酸的活性取决于所评估的菌株。对（美国典型培养物保藏中心11578株及临床分离株）在培养8小时前就出现了杀菌作用。对于所有测试细菌，油树脂和多花含笑酸至少抑制生物膜形成50%。油树脂和多花含笑酸分别对（美国典型培养物保藏中心19039株）和（美国典型培养物保藏中心11578株）表现出最佳活性。将油树脂或多花含笑酸与氯己定联合进行的协同试验未得到有趣的结果。我们检测了德怀尔油树脂、埃佩鲁 - 8(20)-15,18 - 二酸和多花含笑酸对中国仓鼠肺成纤维细胞的细胞毒性。油树脂和多花含笑酸在浓度高于78.1μg/mL时具有细胞毒性，而埃佩鲁 - 8(20)-15,18 - 二酸在浓度高于312.5μg/mL时显示出细胞毒性。总之，油树脂和多花含笑酸是潜在的抗菌剂来源，在不引起细胞毒性的浓度下，对以固着和浮游形式存在的引起原发性牙髓感染和龋齿的细菌具有抗菌作用。
