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黄瓜谷胱甘肽过氧化物酶(GPX)基因家族的全基因组鉴定及其对非生物胁迫的响应

Genome-wide identification of glutathione peroxidase (GPX) gene family and their response to abiotic stress in cucumber.

作者信息

Zhou Yong, Hu Lifang, Ye Shuifeng, Jiang Lunwei, Liu Shiqiang

机构信息

1Department of Biochemistry and Molecular Biology, College of Science, Jiangxi Agricultural University, Nanchang Economic and Technological Development District, Nanchang, 330045 Jiangxi China.

2Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, College of Agronomy, Jiangxi Agricultural University, Nanchang Economic and Technological Development District, Nanchang, 330045 Jiangxi China.

出版信息

3 Biotech. 2018 Mar;8(3):159. doi: 10.1007/s13205-018-1185-3. Epub 2018 Mar 3.

Abstract

Plant glutathione peroxidases (GPXs) are non-heme thiol peroxidases that play vital roles in maintaining HO homeostasis and regulating plant response to abiotic stress. Here, we performed a comparative genomic analysis of the gene family in cucumber (). As a result, a total of 6 genes were identified, which were unevenly located in four out of the seven chromosomes in cucumber genome. Based on the phylogenetic analysis, the genes of cucumber, Arabidopsis and rice could be classified into five groups. Analysis of the distribution of conserved domains of GPX proteins showed that all these proteins contain three highly conserved motifs, as well as other conserved sequences and residues. Gene structure analysis revealed a conserved exon-intron organization pattern of these genes. Through analyzing the promoter regions of genes, many hormone-, stress-, and development-responsive -elements were identified. Moreover, we also investigated their expression patterns in different tissues and developmental stages as well as in response to abiotic stress and x acid (ABA) treatments. The qRT-PCR results showed that the transcripts of genes varied largely under abiotic stress and ABA treatments at different time points. These results demonstrate that cucumber gene family may function in tissue development and plant stress responses.

摘要

植物谷胱甘肽过氧化物酶(GPXs)是一类非血红素硫醇过氧化物酶,在维持过氧化氢(HO)稳态和调节植物对非生物胁迫的响应中发挥着至关重要的作用。在此,我们对黄瓜()中的该基因家族进行了比较基因组分析。结果,共鉴定出6个基因,它们不均匀地分布在黄瓜基因组7条染色体中的4条上。基于系统发育分析,黄瓜、拟南芥和水稻的基因可分为5组。对GPX蛋白保守结构域分布的分析表明,所有这些蛋白都包含三个高度保守的基序,以及其他保守序列和残基。基因结构分析揭示了这些基因保守的外显子-内含子组织模式。通过分析基因的启动子区域,鉴定出许多激素、胁迫和发育响应元件。此外,我们还研究了它们在不同组织和发育阶段以及对非生物胁迫和脱落酸(ABA)处理的表达模式。qRT-PCR结果表明,在不同时间点的非生物胁迫和ABA处理下,基因的转录本有很大差异。这些结果表明,黄瓜基因家族可能在组织发育和植物胁迫响应中发挥作用。

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