Genome-Wide Characterization of Glutathione Peroxidase (GPX) Gene Family in Rapeseed ( L.) Revealed Their Role in Multiple Abiotic Stress Response and Hormone Signaling.

Plant glutathione peroxidases (GPXs) are the main enzymes in the antioxidant defense system that sustain HO homeostasis and normalize plant reaction to abiotic stress conditions. To understand the major roles of the gene family in rapeseed ( L.), for the first time, a genome-wide study identified 25 genes in the rapeseed genome. The phylogenetic analysis discovered that genes were grouped into four major groups (Group I-Group IV) from rapeseed and three closely interrelated plant species. The universal investigation uncovered that the gene experienced segmental duplications and positive selection pressure. Gene structure and motifs examination recommended that most of the genes demonstrated a comparatively well-maintained exon-intron and motifs arrangement within the identical group. Likewise, we recognized five hormones-, four stress-, and numerous light-reactive -elements in the promoters of . Five putative bna-miRNAs from two families were also prophesied, targeting six genes. Gene ontology annotation results proved the main role of in antioxidant defense systems, ROS, and response to stress stimulus. Several genes revealed boosted expression profiles in many developmental tissues/organs, i.e., root, seed, leaf, stem, flower, and silique. The qRT-PCR based expression profiling exhibited that two genes ( and ) were suggestively up-regulated against different hormones (ABA, IAA, and MeJA) and abiotic stress (salinity, cold, waterlogging, and drought) treatments. In short, our discoveries provide a basis for additional functional studies on the genes in future rapeseed breeding programs.