Tian Li, Hou Li, Wang Lixin, Xu Hong, Xiao Jie, Ying Binwu
Department of Laboratory Medicine, Chengdu, P.R. China.
Department of Blood Immunology, Institute of Blood Transfusion, Chinese Academy of Medical Sciences, Chengdu, P.R. China.
Transfusion. 2018 Jun;58(6):1536-1539. doi: 10.1111/trf.14568. Epub 2018 Mar 7.
Anti-E is the most common and important RBC alloantibody in the Chinese population. Several studies have demonstrated that the production of specific RBC alloantibodies is associated with HLA-DRB1 polymorphisms. Considering that the Chinese population has its own unique characteristics of HLA-DRB1 polymorphisms, we investigate whether specific HLA-DRB1 alleles are associated with E immunization in a Chinese population.
The frequencies of HLA-DRB1 phenotypes were compared among 78 patients possessing anti-E and 192 healthy blood donors. HLA-DRB1 genotyping was carried out using sequence-based typing method. The TEPITOPEpan software was used to predict E antigen-derived anchor peptides binding to HLA-DRB1 molecules.
The frequency of HLA-DRB109:01 phenotype was significantly higher in the patients with anti-E than in healthy controls: 76.9% versus 27.6% (odds ratio, 8.7; 95% confidence interval, 4.7-16.2; corrected p value < 0.0034). One E antigen-derived anchor peptide (217WMFWPSVNS225) was predicted to bind three HLA-DRB1 molecules (HLA-DRB104:05, *04:17, and 13:02); however, no anchor peptide was predicted to bind HLA-DRB109:01.
This study indicated that HLA-DRB109:01 phenotype was significantly more prevalent in E-immunized patients than the control group. It suggested that HLA-DRB109:01 molecule might represent a susceptibility phenotype enhancing formation of anti-E alloantibody. Further study would be necessary to identify the anchor peptide responsible for E alloimmunization by stimulation of specific T cells by peptide originating from E antigen.
抗 - E 是中国人群中最常见且重要的红细胞同种抗体。多项研究表明，特定红细胞同种抗体的产生与 HLA - DRB1 基因多态性相关。鉴于中国人群具有自身独特的 HLA - DRB1 基因多态性特征，我们研究特定的 HLA - DRB1 等位基因是否与中国人群中的 E 免疫相关。
比较了 78 例具有抗 - E 的患者和 192 名健康献血者中 HLA - DRB1 表型的频率。采用基于序列的分型方法进行 HLA - DRB1 基因分型。使用 TEPITOPEpan 软件预测与 HLA - DRB1 分子结合的 E 抗原衍生锚定肽。
抗 - E 患者中 HLA - DRB109:01 表型的频率显著高于健康对照组：分别为 76.9% 和 27.6%（优势比，8.7；95% 置信区间，4.7 - 16.2；校正 p 值 < 0.0034）。预测一个 E 抗原衍生的锚定肽（217WMFWPSVNS225）可与三个 HLA - DRB1 分子（HLA - DRB104:05、*04:17 和 13:02）结合；然而，未预测到有锚定肽可与 HLA - DRB109:01 结合。
本研究表明，HLA - DRB109:01 表型在 E 免疫患者中比对照组更为普遍。这表明 HLA - DRB109:01 分子可能代表一种易感性表型，增强了抗 - E 同种抗体的形成。有必要进一步研究以确定通过源自 E 抗原的肽刺激特定 T 细胞而导致 E 同种免疫的锚定肽。
