Duan Wen-Xiu, Wu Zi-Jian, Hu Ling, Song Xiao-Ge, Gong Chang-Ping, He Lu, Liu Lei, Yu Qing, Wang Jie
School of Acupuncture and Othopedics-Traumatology, Anhui University of Traditional Chinese Medicine, Hefei 230038, China; Department of Rehabilitation, Anhui Provincial Hospital, Hefei 230001.
Institute of Acupuncture and Meridian, Anhui Academy of Traditional Chinese Medicine, Hefei 230038.
Zhen Ci Yan Jiu. 2018 Feb 25;43(2):98-103. doi: 10.13702/j.1000-0607.170854.
To study the effect of moxa-smoke inhaling on the respiratory system, so as to provide experimental data and theoretical basis for evaluating the safety of moxa-smoke inhaling during moxibustion treatment.
A total of 48 SD rats were randomized into control, low, medium and high moxa-smoke-concentration groups (＝12 in each group). The low, medium and high concentrations of smoke were controlled in (0.11±0.05) mg/m (0.23±0.05) mg/m and (0.53±0.05) mg/m respectively in each of 3 glass boxes (with reference to the level of PM 2.5). The smoking was conducted 4 hours each time, twice a day for 100 days. The normal group did not receive any moxa-smoke inhaling. The histopathological changes of lung and bronchial tissues were detected by H．E. stainning, and the contents of TNF-α and IL-1 β of plasma, bronchoalveolar la-vage fluid (BALF) and lung tissue detected by ELISA. The levels of forced vital capacity (FVC), forced expiratory volume (FEV), FEV 0.3/FVC (0.3＝ the 0.3 second), maximal mid-expiratory flow rate(MMEF), peak expiratory flow(PEF) were detected by animal pulmonary function analysis system.
After 100 days' moxa-smoke inhaling, the contents of TNF-α in the plasma, BALF and lung tissues and IL-1 β in the lung tissue of the low, medium and high concentration moxa-smoke groups, and IL-1 β in the plasma and BALF of the medium and high concentration groups were significantly increased relevant to the control group (<0.05, <0.01). H．E. stain showed various inflammatory changes in the lungs and trachea tissues, including obvious fusion of pulmonary alveoli, lymphocyte infiltration, increase of capillary permeability, red blood cell exudation, etc. in the high concentration group, these situations were milder in the medium concentration group and were not obvious in the low concentration group. Compared with the control group, there were no significant changes in the FVC, FEV, FEV 0.3/FVC, MMEF and PEF of lung function in the three concentration groups (> 0.05)．.
Long term inhalation of high concentration of moxa-smoke may lead to inflammatory injury in the lung and bronchial tissues but has no significant effect on the respiratory function in rats. Nevertheless, a good air-ventilation during moxibustion in a treatment room is necessary.
研究艾灸烟雾吸入对呼吸系统的影响，为评估艾灸治疗过程中艾灸烟雾吸入的安全性提供实验数据和理论依据。
将48只SD大鼠随机分为对照组、低、中、高艾灸烟雾浓度组（每组12只）。参照PM 2.5水平，在3个玻璃箱中分别将低、中、高浓度烟雾控制在（0.11±0.05）mg/m、（0.23±0.05）mg/m和（0.53±0.05）mg/m。每次烟熏4小时，每天2次，共100天。正常组不进行任何艾灸烟雾吸入。通过苏木精-伊红（H.E.）染色检测肺和支气管组织的组织病理学变化，采用酶联免疫吸附测定（ELISA）法检测血浆、支气管肺泡灌洗液（BALF）和肺组织中肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的含量。用动物肺功能分析系统检测用力肺活量（FVC）、用力呼气量（FEV）、FEV 0.3/FVC（0.3为第0.3秒）、最大呼气中期流速（MMEF）、呼气峰值流速（PEF）水平。
艾灸烟雾吸入100天后，低、中、高浓度艾灸烟雾组血浆、BALF和肺组织中TNF-α的含量以及中、高浓度组肺组织中IL-1β的含量，与对照组相比均显著升高（P<0.05，P<0.01）；中、高浓度组血浆和BALF中IL-1β的含量与对照组相比也显著升高（P<0.05，P<0.01）。H.E.染色显示肺和气管组织出现各种炎症变化，高浓度组包括肺泡明显融合、淋巴细胞浸润、毛细血管通透性增加、红细胞渗出等，中浓度组这些情况较轻，低浓度组不明显。与对照组相比，三个浓度组肺功能的FVC、FEV、FEV 0.3/FVC、MMEF和PEF均无显著变化（P>0.05）。
长期吸入高浓度艾灸烟雾可能导致肺和支气管组织发生炎症损伤，但对大鼠呼吸功能无显著影响。尽管如此，治疗室内艾灸时保持良好通风是必要的。
