Zhou Jing-Ying, Lai Yi-Tian, Ding Pan-Ting, Liu Mi, Li Nan, Zhang Guo-Shan, Qiu Ran-Ran
Graduate School of Hunan University of Chinese Medicine, Changsha 410208, China.
College of Acupuncture-moxibustion-Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha 410208.
Zhen Ci Yan Jiu. 2022 Nov 25;47(11):969-74. doi: 10.13702/j.1000-0607.20220121.
To investigate the role of moxibustion of "Feishu" (BL13)，"Tianshu" (ST25) for asthma by simultaneously treating lung and intestine (i.e., treating both lung and intestine at the same time) in asthmatic rats.
A total of 48 SD rats were randomly divided into normal, model, lung treatment and joint-treatment of lung and intestine (joint-treatment) groups, with 12 rats in each. The asthma model was established by subcutaneous (bilateral back and inguinal regions) and intraperitoneal injection of mixture solution of albumin and Aluminium Hydroxide gel (on day 1, and 9) and followed by inhalation of atomized 1% ovalbumin (on day 15, 20 min each time, once daily for 1 week). Moxibustion was applied to bilateral BL13 for rats of the lung treatment group or bilateral BL13 and ST25 for rats of the joint-treatment group. One hour after the intervention, the rats in the later three groups were separately given nebulized 1% ovalbumin solution inhalation for 20 min. The treatments were conducted once daily for 14 consecutive days. After intervention, the lung functions including the forced expiratory flow 25% (FEF 25%), maximal mid-expiratory flow (MMEF), dynamic lung compliance (Cdyn), forced expiratory volume/ forced vital capacity (FEV/FVC), peak expiratory flow (PEF), and lung resistance (RL) were measured by using a small animal lung function detector, and pathological changes and collagen deposition in the lung tissues were observed by H.E. and Masson staining, separately. The levels of interleukin (IL)-17, IL-4, IL-13, IL-33, IL-5, leukotriene (LT) and thymic stromal lymphocyte (TSLP) in the lung tissue were measured by ELISA.
Compared with the normal group, the FEF 25%, MMEF， Cdyn， FEV/FVC and PEF were significantly decreased (<0.05, <0.01)， and the pulmonary RL, collagen deposition, and contents of IL-17, IL-4, IL-13, IL-33, IL-5, TSLP and LT were notably increased (<0.05, <0.01) in the model group. After intervention, the MMEF and Cdyn in the lung treatment group, PEF, MMEF, Cdyn, FEV/FVC, FEF 25% in the joint-treatment group, were markedly increased (<0.05, <0.01), whereas the collagen deposition, IL-17, IL-4 and TSLP in both the lung treatment and joint-treatment groups, RL, IL-13, IL-33, IL-5 and LT in the joint-treatment group were considerably down-regulated (<0.05, <0.01). The effects of the joint treatment were apparently superior to those of lung treatment in down-regulating the contents of TSLP and LT (<0.05, <0.01). H.E. staining showed thickened alveolar wall, infiltration of a large number of inflammatory cells in the bronchus of the model group, which was relatively milder in the joint-treatment group.
"Joint treatment of lung and intestine" with moxibustion is superior to "lung treatment" alone in ameliorating the lung function and mitigating airway inflammation in rats with asthma.
通过对哮喘大鼠同时进行肺肠同治，探讨艾灸“肺俞”（BL13）、“天枢”（ST25）对哮喘的作用。
将48只SD大鼠随机分为正常组、模型组、肺治疗组和肺肠同治组，每组12只。采用皮下（双侧背部和腹股沟区）及腹腔注射白蛋白与氢氧化铝凝胶混合液（第1天和第9天），随后雾化吸入1%卵清蛋白（第15天开始，每次20分钟，每日1次，共1周）的方法建立哮喘模型。对肺治疗组大鼠艾灸双侧BL13，对肺肠同治组大鼠艾灸双侧BL13和ST25。干预1小时后，后三组大鼠分别雾化吸入1%卵清蛋白溶液20分钟。每日治疗1次，连续14天。干预后，使用小动物肺功能检测仪检测肺功能，包括用力呼气流量25%（FEF 25%）、最大呼气中期流量（MMEF）、动态肺顺应性（Cdyn）、用力呼气量/用力肺活量（FEV/FVC）、呼气峰值流量（PEF）和肺阻力（RL），并分别采用苏木精-伊红（H.E.）染色和Masson染色观察肺组织的病理变化及胶原沉积情况。采用酶联免疫吸附测定（ELISA）法检测肺组织中白细胞介素（IL）-17、IL-4、IL-13、IL-33、IL-5、白三烯（LT）和胸腺基质淋巴细胞生成素（TSLP）的水平。
与正常组比较，模型组FEF 25%、MMEF、Cdyn、FEV/FVC和PEF显著降低（<0.05，<0.01），肺组织RL、胶原沉积以及IL-17、IL-4、IL-13、IL-33、IL-5、TSLP和LT含量显著升高（<0.05，<0.01）。干预后，肺治疗组MMEF和Cdyn、肺肠同治组PEF、MMEF、Cdyn、FEV/FVC、FEF 25%显著升高（<0.05，<0.01），而肺治疗组和肺肠同治组的胶原沉积、IL-17、IL-4和TSLP，肺肠同治组的RL、IL-13、IL-33、IL-5和LT均显著下调（<0.05，<0.01）。肺肠同治组在下调TSLP和LT含量方面的效果明显优于肺治疗组（<0.05，<0.01）。H.E.染色显示，模型组肺泡壁增厚，支气管内大量炎性细胞浸润，肺肠同治组相对较轻。
艾灸“肺肠同治”在改善哮喘大鼠肺功能、减轻气道炎症方面优于单纯“肺治疗”。
