Department of Zoology, Sri Venkateswara College, University of Delhi, Dhaula Kuan, New Delhi, India 110021.
Department of Zoology, Kalindi College, University of Delhi, Delhi, 110008, India.
Fish Shellfish Immunol. 2018 May;76:324-332. doi: 10.1016/j.fsi.2018.03.011. Epub 2018 Mar 5.
The laboratory acclimatized Clarias gariepinus (80 ± 10 g) were divided into six groups and five subgroups each containing 10 fish. A fish feed was reconstituted by adding 33% powder of Leucaena leucocephala seed in place of fish trash. Group B, C and E were fed on reconstituted feed and group A, D and F were fed on artificial feed containing animal protein for 7 days prior to start of experiments. Then Group B was challenged with BSA while other groups were challenged with Vibrio harveyi (Group C, D) and Pseudomonas aeruginosa (Group E, F). Group A was used as negative control (not challenged with antigen). The fish were challenged on weekly intervals till 28th day. Blood was collected from one subgroup of each group on day 7, 14, 21 & 28 and finally sacrificed on day 35. Change in body weight, liver function tests (SGOT, SGPT) and serum ALP levels were monitored. The phagocytic index, percentage phagocytosis and nitric oxide levels were measured in macrophages isolated from spleen and head kidney. The levels of total fish immunoglobulin were also measured following indirect ELISA. The results showed improved immune response in fish fed on 33% L. leucocephala pod seed reconstituted feed; however their specific growth rate was low.
实验室驯化的 Gariepinus gariepinus（80±10g）被分为六组，每组五个亚组，每组 10 条鱼。鱼饲料通过添加 33%的 Leucaena leucocephala 种子粉来替代鱼饲料进行重构。组 B、C 和 E 以重构饲料喂养，组 A、D 和 F 在实验开始前 7 天以含有动物蛋白的人工饲料喂养。然后组 B 用 BSA 进行挑战，而其他组用哈维氏弧菌（组 C、D）和铜绿假单胞菌（组 E、F）进行挑战。组 A 作为阴性对照（未用抗原挑战）。每周对鱼进行一次挑战，直到第 28 天。在第 7、14、21 和 28 天，从每组的一个亚组收集血液，最后在第 35 天处死。监测体重变化、肝功能测试（SGOT、SGPT）和血清 ALP 水平。在分离自脾脏和头肾的巨噬细胞中测量吞噬指数、吞噬百分比和一氧化氮水平。还通过间接 ELISA 测量总鱼免疫球蛋白的水平。结果表明，喂食 33%L. leucocephala 豆荚种子重构饲料的鱼的免疫反应得到改善；然而，它们的特定生长率较低。
