Department of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Korea.
Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 41566, Korea.
Int J Mol Sci. 2018 Mar 7;19(3):760. doi: 10.3390/ijms19030760.
In this study, the antimelanogenic effect of an ethyl acetate fraction of Vent. seeds (OISEA) and its underlying mechanisms in melan-a cells were investigated. Antimelanogenesis activity was confirmed by assessing inhibition of tyrosinase activity and melanin content in the cells. Both transcriptional and translational expression of microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase related protein-1 and 2 (TYRP-1 and TYRP-2), were also examined. The results depicted that pretreatment of OISEA significantly inhibits not only tyrosinase activity, but melanin production and intracellular tyrosinase activity. By repressing the expression of tyrosinase, TYRP-1, TYRP-2, and MITF, OISEA interrupted melanin production. Additionally, OISEA interfered with the phosphorylation of p38, extracellular signal-regulated kinase 1/2 (ERK1/2), and c-Jun N-terminal kinase (JNK), with the reversal of OISEA-induced melanogenesis inhibition after treatment with the specific inhibitors SB239063, U0126, and SP600125. Overall, these results suggest that OISEA can stimulate p38, ERK1/2, JNK phosphorylation, and subsequent suppression of melanin, leading to the inhibition of melanogenic enzymes and melanin production, possibly owing to the presence of polyphenolic compounds.
在这项研究中，我们研究了八角茴香种子的乙酸乙酯部位（OISEA）的抗黑色素生成作用及其在黑素-a 细胞中的潜在机制。通过评估细胞中酪氨酸酶活性和黑色素含量的抑制来证实抗黑色素生成活性。还检查了小眼畸形相关转录因子（MITF）、酪氨酸酶和酪氨酸酶相关蛋白-1 和 2（TYRP-1 和 TYRP-2）的转录和翻译表达。结果表明，OISEA 的预处理不仅显著抑制了酪氨酸酶活性，而且还抑制了黑色素的产生和细胞内酪氨酸酶活性。通过抑制酪氨酸酶、TYRP-1、TYRP-2 和 MITF 的表达，OISEA 中断了黑色素的产生。此外，OISEA 干扰了 p38、细胞外信号调节激酶 1/2（ERK1/2）和 c-Jun N-末端激酶（JNK）的磷酸化，并且在用特异性抑制剂 SB239063、U0126 和 SP600125 处理后，OISEA 诱导的黑色素生成抑制作用得到逆转。总的来说，这些结果表明，OISEA 可以刺激 p38、ERK1/2、JNK 磷酸化，随后抑制黑色素，从而抑制黑色素生成酶和黑色素的产生，这可能是由于存在多酚化合物。
