Zhao Peijun, Park Na Hyun, Alam Md Badrul, Lee Sang-Han
Department of Food Science and Biotechnology, Graduate School, Kyungpook National University, Daegu 41566, Korea.
Department of Food Science and Technology, Henan Agricultural University, Zhengzhou 450046, China.
Antioxidants (Basel). 2022 Mar 21;11(3):599. doi: 10.3390/antiox11030599.
The anti-graying effect of the hexane fraction of Fuzhuan brick tea is investigated in Melan-A cells and C57BL/6 mice. As a result, it is found that reactive oxygen species-induced damage is associated with the reduction of melanogenesis in hair bulb melanocytes when reactive oxygen species generation in Melan-A cells occurred. The results revealed that the hexane fraction of Fuzhuan brick tea could remarkably reduce reactive oxygen species generation in Melan-A cells; meanwhile, it could increase the cellular tyrosinase and melanin content, as well as up-regulate the expression of tyrosinase, tyrosinase related protein-1, tyrosinase related protein-2, and microphthalmia-associated transcription factor, and activate the MAP-kinase pathway through activating the phosphorylation of p38 c-Jun N terminal kinase/extracellular signal-regulated kinase. Furthermore, high-pressure liquid chromatography analysis reveals that the tea's major ingredients in hexane fraction include gallic acid, theaflavin, theobromine, caffeine, epicatechin, and quercetin. Together, the current results suggest that Fuzhuan brick tea proves to protect from the damage of hydroquinone, which induces hair pigment loss.
在黑素-A细胞和C57BL/6小鼠中研究了茯砖茶正己烷提取物的抗白发作用。结果发现，当黑素-A细胞中活性氧生成时，活性氧诱导的损伤与毛球黑素细胞中黑素生成的减少有关。结果表明，茯砖茶正己烷提取物可显著减少黑素-A细胞中活性氧的生成；同时，它可以增加细胞酪氨酸酶和黑色素含量，上调酪氨酸酶、酪氨酸酶相关蛋白-1、酪氨酸酶相关蛋白-2和小眼畸形相关转录因子的表达，并通过激活p38 c-Jun N末端激酶/细胞外信号调节激酶的磷酸化来激活丝裂原活化蛋白激酶途径。此外，高压液相色谱分析表明，该茶正己烷提取物中的主要成分包括没食子酸、茶黄素、可可碱、咖啡因、表儿茶素和槲皮素。总之，目前的结果表明，茯砖茶被证明可以保护免受诱导毛发色素流失的对苯二酚的损害。
