Laboratório de Pesquisa em Farmacologia Bioquímica - LaFarBio - Grupo de Pesquisa em Neurobiotecnologia, CCQFA - Universidade Federal de Pelotas, UFPel, P.O. Box 354, 96010-900 Pelotas, RS, Brazil.
Laboratório de Tecnologia e Desenvolvimento de Compósitos e Materiais Poliméricos (LaCoPol), Centro de Ciências Químicas, Farmacêuticas e de Alimentos, Universidade Federal de Pelotas (UFPel), 96010-900 Pelotas, RS, Brazil.
Mater Sci Eng C Mater Biol Appl. 2018 Mar 1;84:90-98. doi: 10.1016/j.msec.2017.11.026. Epub 2017 Nov 23.
This study investigated the main effects of the oral treatment with 7-chloro-4-phenylselanyl quinoline (4-PSQ) on symptoms, inflammatory and oxidative parameters in an atopic dermatitis (AD) model in BALB/c mice. In addition, the possibility of antioxidant property of 4-PSQ improves the potential of a biofilm (based on chitosan/poly(vinyl alcohol) (PVA)/ bovine bone powder (BBP)) for the treatment of AD-like skin lesions was evaluated. 2,4-Dinitrochlorobenzene (DNCB) was applied to the dorsal skin on days 1-3 for sensitization. Mice were challenged with DNCB on the ear (on days 14-29) and dorsal skin (on days 14, 17, 20, 23, 26, and 29) and treated with 4-PSQ, dexamethasone, biofilm (biofilm sample without 4-PSQ) or 4-PSQ-loaded biofilms. On the day 30, skin severity scores and scratching behavior were determined. After that, animals were sacrificed, and ears and dorsal skin were removed for determination of inflammatory and oxidative parameters. DNCB induced the skin lesions, scratching behavior and ear swelling, increased myeloperoxidase (MPO) activity (ear and back) and reactive species (RS) levels (back). 4-PSQ, 4-PSQ-loaded biofilms and biofilm treatments ameliorated skin severity scores, scratching behavior and inflammatory response induced by DNCB. 4-PSQ and 4-PSQ-loaded biofilm treatments partially protected against the increase in the RS levels induced by DNCB. Our results revealed that the incorporation of 4-PSQ improved the therapeutic effect of the biofilm. The efficacy of 4-PSQ in treating AD-like lesions was similar or better than dexamethasone. In summary, 4-PSQ has a potential therapeutic advantage in the treatment and management of AD.
本研究旨在探讨口服 7-氯-4-苯硒基喹啉（4-PSQ）对 BALB/c 小鼠特应性皮炎（AD）模型症状、炎症和氧化参数的主要影响。此外，还评估了 4-PSQ 的抗氧化特性是否可以改善基于壳聚糖/聚乙烯醇（PVA）/牛骨粉（BBP）的生物膜治疗 AD 样皮肤损伤的潜力。在第 1-3 天，背部皮肤涂抹 2,4-二硝基氯苯（DNCB）进行致敏。在第 14-29 天，用 DNCB 于耳部和背部进行攻毒，在第 14、17、20、23、26 和 29 天，用 4-PSQ、地塞米松、生物膜（不含 4-PSQ 的生物膜样品）或负载 4-PSQ 的生物膜进行治疗。第 30 天，测定皮肤严重程度评分和搔抓行为。之后处死动物，取耳和背部皮肤测定炎症和氧化参数。DNCB 诱导皮肤病变、搔抓行为和耳部肿胀，增加髓过氧化物酶（MPO）活性（耳部和背部）和活性氧（RS）水平（背部）。4-PSQ、负载 4-PSQ 的生物膜和生物膜治疗可改善 DNCB 诱导的皮肤严重程度评分、搔抓行为和炎症反应。4-PSQ 和负载 4-PSQ 的生物膜治疗部分保护 DNCB 诱导的 RS 水平升高。我们的研究结果表明，4-PSQ 的加入提高了生物膜的治疗效果。4-PSQ 治疗 AD 样病变的疗效与地塞米松相似或优于地塞米松。综上所述，4-PSQ 在 AD 的治疗和管理方面具有潜在的治疗优势。
