School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China; Department of Traditional Chinese Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, China.
School of Traditional Chinese Medicine, Southern Medical University, Guangzhou, Guangdong 510515, China.
J Ethnopharmacol. 2018 Aug 10;222:1-10. doi: 10.1016/j.jep.2018.04.032. Epub 2018 Apr 24.
Si-Ni-San (SNS) is a well-known decoction in traditional Chinese medicine. Although studies have indicated that the anti-inflammatory and anti-allergic properties of SNS and its components can account for their therapeutic effects, the role and mechanism of SNS in treating skin dysfunction remain unclear.
Atopic dermatitis (AD), a disorder known for its prevalence in infants and adults, severely influences the quality of life of affected patients. In this study, we aimed to investigate the anti-inflammatory and immune response modulations of SNS in 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin dysfunction.
Dermatitis was induced in Kunming mice by the topical application of DNCB. SNS or dexamethasone (positive control) was topically applied every day over the course of the 21-day study. The following were assessed: dermatitis severity scores; ear and dorsal skin haematoxylin and eosin staining; interleukin (IL)- 1α, IL-1β, IL-2, IL-4, IL-6, and tumour necrosis factor (TNF)-α cytokine levels in the serum; spleen index; spleen CD4 + /CD8 + T lymphocyte ratio; and phosphorylation levels of mitogen-activated protein kinases (MAPKs- p38, extracellular signal-regulated kinase (ERK), and c-Jun N-terminal kinase (JNK)), IκB-α, and nuclear factor (NF)-κB (p65) in skin lesions.
SNS significantly alleviated the symptoms of AD-like lesions induced by DNCB, decreased the infiltration of inflammatory cells in the ear and dorsal tissues, suppressed the increased cytokine levels in the serum, reduced the CD4 + /CD8 +T lymphocyte ratio in the spleen, and downregulated the activation of MAPKs, IκB-α, and NF-κB (p65) in the dorsal skin. The effects were similar to those of dexamethasone.
SNS alleviated the DNCB-induced AD-like skin dysfunction in mice through anti-inflammatory and immune system modulation, indicating that SNS shows potential for AD treatment in clinical settings.
四逆散（SNS）是一种著名的中药方剂。虽然研究表明 SNS 及其成分的抗炎和抗过敏特性可以解释其治疗效果，但 SNS 治疗皮肤功能障碍的作用和机制尚不清楚。
特应性皮炎（AD）是一种在婴儿和成人中普遍存在的疾病，严重影响了受影响患者的生活质量。在这项研究中，我们旨在研究 SNS 对 2,4-二硝基氯苯（DNCB）诱导的 AD 样皮肤功能障碍的抗炎和免疫反应调节作用。
通过局部应用 DNCB 诱导昆明小鼠皮炎。在 21 天的研究过程中，每天局部应用 SNS 或地塞米松（阳性对照）。评估以下指标：皮炎严重程度评分；耳和背部皮肤苏木精和伊红染色；血清中白细胞介素（IL）-1α、IL-1β、IL-2、IL-4、IL-6 和肿瘤坏死因子（TNF）-α细胞因子水平；脾指数；脾 CD4+/CD8+T 淋巴细胞比值；皮肤病变中丝裂原活化蛋白激酶（MAPKs-p38、细胞外信号调节激酶（ERK）和 c-Jun N 末端激酶（JNK））、IκB-α 和核因子（NF）-κB（p65）的磷酸化水平。
SNS 显著缓解了 DNCB 诱导的 AD 样病变的症状，减少了耳部和背部组织中炎症细胞的浸润，抑制了血清中细胞因子水平的升高，降低了脾中 CD4+/CD8+T 淋巴细胞的比值，并下调了背部皮肤中 MAPKs、IκB-α 和 NF-κB（p65）的激活。这些作用与地塞米松相似。
SNS 通过抗炎和免疫系统调节缓解了 DNCB 诱导的小鼠 AD 样皮肤功能障碍，表明 SNS 在临床环境中具有治疗 AD 的潜力。
