Lipps Christoph, Klein Franziska, Wahlicht Tom, Seiffert Virginia, Butueva Milada, Zauers Jeannette, Truschel Theresa, Luckner Martin, Köster Mario, MacLeod Roderick, Pezoldt Jörn, Hühn Jochen, Yuan Qinggong, Müller Peter Paul, Kempf Henning, Zweigerdt Robert, Dittrich-Breiholz Oliver, Pufe Thomas, Beckmann Rainer, Drescher Wolf, Riancho Jose, Sañudo Carolina, Korff Thomas, Opalka Bertram, Rebmann Vera, Göthert Joachim R, Alves Paula M, Ott Michael, Schucht Roland, Hauser Hansjörg, Wirth Dagmar, May Tobias
Model Systems for Infection and Immunity, HZI - Helmholtz Centre for Infection Research, Inhoffenstr. 7, 38124, Braunschweig, Germany.
Experimental Cardiology, Justus-Liebig University Giessen, Aulweg 129, 35392, Giessen, Germany.
Nat Commun. 2018 Mar 8;9(1):994. doi: 10.1038/s41467-018-03408-4.
Fundamental research and drug development for personalized medicine necessitates cell cultures from defined genetic backgrounds. However, providing sufficient numbers of authentic cells from individuals poses a challenge. Here, we present a new strategy for rapid cell expansion that overcomes current limitations. Using a small gene library, we expanded primary cells from different tissues, donors, and species. Cell-type-specific regimens that allow the reproducible creation of cell lines were identified. In depth characterization of a series of endothelial and hepatocytic cell lines confirmed phenotypic stability and functionality. Applying this technology enables rapid, efficient, and reliable production of unlimited numbers of personalized cells. As such, these cell systems support mechanistic studies, epidemiological research, and tailored drug development.
个性化医疗的基础研究和药物开发需要来自特定遗传背景的细胞培养物。然而，从个体获取足够数量的真实细胞是一项挑战。在此，我们提出一种克服当前限制的快速细胞扩增新策略。利用一个小基因文库，我们扩增了来自不同组织、供体和物种的原代细胞。确定了能够可重复创建细胞系的细胞类型特异性方案。对一系列内皮细胞和肝细胞系的深入表征证实了其表型稳定性和功能。应用这项技术能够快速、高效且可靠地生产无限数量的个性化细胞。因此，这些细胞系统支持机制研究、流行病学研究和定制药物开发。
