Department of Pathology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, People's Republic of China.
Department of Pathology, Affiliated People' Hospital of Jiangsu University, Zhenjiang, Jiangsu, People's Republic of China.
Biosci Rep. 2018 Apr 20;38(2). doi: 10.1042/BSR20171193. Print 2018 Apr 26.
Pseudogenes were once regarded as transcriptionally inactive and without specific molecular function. However, current evidence shows that pseudogene-derived long non-coding RNAs (lncRNAs) may be crucial regulators of human cancer development, including gastric cancer (GC). In the present study, we report that a pseudogene-derived lncRNA named surfactant associated 1, pseudogene (SFTA1P), which is 693-nt long, was significantly down-regulated in GC tissues compared with that in the adjacent normal tissues. In addition, decreased SFTA1P expression was strongly correlated with advanced tumor lymph node metastasis (TNM) stage, larger tumor size, lymphatic metastasis, and poor prognosis of patients with GC. Moreover, gain-of-function experiments revealed that the overexpression of SFTA1P inhibits cell proliferation, migration, and invasion, thus verifying the tumor inhibitory role of SFTA1P in GC. Furthermore, we investigated the potential action mechanism of SFTA1P. Our results showed that down-regulation of SFTA1P may be associated with decreased TP53 expression. In summary, our work suggests that the pseudogene-derived lncRNA SFTA1P functions as a tumor suppressor in GC and thus may act as a potential diagnostic and therapeutic target of GC.
假基因曾被认为是转录失活的，没有特定的分子功能。然而，目前的证据表明，假基因衍生的长非编码 RNA（lncRNA）可能是人类癌症发展的关键调节剂，包括胃癌（GC）。在本研究中，我们报告了一个假基因衍生的长非编码 RNA，命名为表面活性剂相关蛋白 1 假基因（SFTA1P），其长度为 693 个核苷酸，在 GC 组织中明显下调，与相邻正常组织相比。此外，SFTA1P 表达的降低与 GC 患者的晚期肿瘤淋巴结转移（TNM）分期、更大的肿瘤大小、淋巴转移和不良预后密切相关。此外，功能获得实验表明，SFTA1P 的过表达抑制细胞增殖、迁移和侵袭，从而验证了 SFTA1P 在 GC 中的肿瘤抑制作用。此外，我们研究了 SFTA1P 的潜在作用机制。我们的结果表明，SFTA1P 的下调可能与 TP53 表达的降低有关。总之，我们的工作表明，假基因衍生的 lncRNA SFTA1P 在 GC 中作为肿瘤抑制因子发挥作用，因此可能成为 GC 的潜在诊断和治疗靶点。
