Servicio General de Apoyo a la Investigación (SEGAI), Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez s/n, 38206, San Cristóbal de La Laguna, Spain.
Departamento de Química, Unidad Departamental de Química Analítica, Facultad de Ciencias, Universidad de La Laguna (ULL), Avda. Astrofísico Fco. Sánchez s/n, 38206, San Cristóbal de La Laguna, Spain.
Anal Bioanal Chem. 2018 Sep;410(22):5617-5628. doi: 10.1007/s00216-018-0977-y. Epub 2018 Mar 9.
In this work, a new method has been developed for the determination of 14 phthalic acid esters (i.e., benzylbutyl phthalate (BBP), bis-2-n-butoxyethyl phthalate (DBEP), dibutyl phthalate (DBP), dicyclohexyl phthalate (DCHP), bis-2-ethoxyethyl phthalate (DEEP), diethyl phthalate (DEP), diisodecyl phthalate (DIDP), diisononyl phthalate (DINP), bis-isopentyl phthalate (DIPP), bis (2-methoxyethyl) phthalate (DMEP), dimethyl phthalate (DMP), di-n-octyl phthalate (DNOP), bis-n-pentyl phthalate (DNPP), dipropyl phthalate (DPP)) and one adipate (bis (2-ethylhexyl) adipate (DEHA)) in different baby foods. Separation was carried out by gas chromatography triple quadrupole tandem mass spectrometry while the previous extraction of the samples was carried out using the QuEChERS method. The methodology was validated for four baby food samples (two fruit compotes of different compositions and two meat and fish purees with vegetables) using dibutyl phthalate-3,4,5,6-d (DBP-d) as internal standard. Determination coefficients (R) of matrix-matched calibration curves were above 0.9922 in all cases while relative recovery values ranged between 70 and 120%, with relative standard deviation values below 19%. The limits of quantification of the method ranged between 0.03 and 1.11 μg/kg. Finally, the analysis of commercially available samples was carried out finding the presence of BBP, DEHA, DEP, DIDP, and DPP in some of the studied samples.
在这项工作中，开发了一种新的方法来测定 14 种邻苯二甲酸酯（即邻苯二甲酸苄丁酯（BBP）、邻苯二甲酸二（2-正丁氧基）乙酯（DBEP）、邻苯二甲酸二丁酯（DBP）、邻苯二甲酸二环己酯（DCHP）、邻苯二甲酸二（2-乙氧基）乙酯（DEEP）、邻苯二甲酸二乙酯（DEP）、邻苯二甲酸二异癸酯（DIDP）、邻苯二甲酸二异壬酯（DINP）、邻苯二甲酸二异戊酯（DIPP）、邻苯二甲酸二（2-甲氧基乙基）酯（DMEP）、邻苯二甲酸二甲酯（DMP）、邻苯二甲酸二正辛酯（DNOP）、邻苯二甲酸二正戊酯（DNPP）、邻苯二甲酸二丙酯（DPP））和一种己二酸酯（邻苯二甲酸二（2-乙基己基）酯（DEHA））在不同婴儿食品中的含量。通过气相色谱三重四极杆串联质谱法进行分离，而样品的前处理采用 QuEChERS 方法。使用邻苯二甲酸二丁酯-3,4,5,6-d（DBP-d）作为内标，对四种婴儿食品样品（两种不同成分的水果混合汁和两种蔬菜肉鱼泥）进行了方法验证。在所有情况下，基质匹配校准曲线的决定系数（R）均高于 0.9922，相对回收率在 70%至 120%之间，相对标准偏差值低于 19%。该方法的定量限范围在 0.03 至 1.11μg/kg 之间。最后，对市售样品进行了分析，发现一些研究样品中存在 BBP、DEHA、DEP、DIDP 和 DPP。
