School of Biomedical and Biological Sciences, University of Plymouth, Drake Circus, Plymouth, PL4 8AA, UK.
Plymouth University Peninsula Schools of Medicine and Dentistry, University of Plymouth, John Bull Building, Tamar Science Park, Plymouth, PL6 8BU, UK.
J Biomed Mater Res B Appl Biomater. 2018 Apr;106(3):1038-1051. doi: 10.1002/jbm.b.33917. Epub 2017 May 10.
Patients with facial prostheses suffer from yeast, Candida albicans, infections. This study aimed to determine the biocompatibility and antifungal properties of silicone facial prostheses coated with silver nanoparticles (Ag NPs) in vitro. Medical grade silicone discs were coated with 5 and 50 mg L dispersions of either Ag NPs or AgNO . Coatings were fully characterized using scanning electron microscopy and energy dispersive X-ray spectroscopy. The biocompatibility was examined using human dermal fibroblasts (Hs68), whereas antifungal efficacy was tested against C. albicans (NCPF-3179). The fibroblast viability was assessed by measuring lactate dehydrogenase (LDH) activity, protein content and tissue electrolytes. There were no effects on the LDH activity of fibroblast cell homogenates, and leak of LDH activity into external media remained low (0.1-0.2 IU mL ). Sublethal effects of Ag NP coatings on membrane permeability/ion balance was not observed, as measured by stable homogenate Na and K concentrations. Some Ag (13 mg L ) was detected from the AgNO coatings in the media, but total Ag remained below detection limit (<1.2 µg L ) for the Ag NP coatings; indicating the latter were stable. When fibroblasts grown on silver coatings were challenged with C. albicans, the Ag NP coating was effective at preventing fungal growth as measured by ethanol production by the yeast, and without damaging the fibroblasts. Ethanol production decreased from 43.2 ± 25.02 in controls to 3.6 µmol mL in all the silver treatments. Data shows that silicone prosthetic materials coated with Ag NPs are biocompatible with fibroblast cells in vitro and show antifungal properties. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1038-1051, 2018.
患有面部假体的患者会遭受酵母菌，白色念珠菌的感染。本研究旨在体外确定涂有银纳米粒子（Ag NPs）的硅橡胶面部假体的生物相容性和抗真菌特性。医用级硅酮圆盘用 5 和 50 mg/L 的 Ag NPs 或 AgNO 分散液进行涂覆。使用扫描电子显微镜和能量色散 X 射线能谱对涂层进行了充分的表征。使用人真皮成纤维细胞（Hs68）检查了生物相容性，而抗真菌功效则针对白色念珠菌（NCPF-3179）进行了测试。通过测量乳酸脱氢酶（LDH）活性，蛋白质含量和组织电解质来评估成纤维细胞的活力。成纤维细胞细胞匀浆的 LDH 活性没有影响，并且 LDH 活性向外部介质的泄漏仍然很低（0.1-0.2 IU/mL）。Ag NP 涂层对膜通透性/离子平衡的亚致死作用没有观察到，因为匀浆 Na 和 K 浓度稳定。在培养基中从 AgNO 涂层中检测到 13mg/L 的 Ag，但对于 Ag NP 涂层，总 Ag 仍低于检测限（<1.2μg/L）;表明后者是稳定的。当在银涂层上生长的成纤维细胞受到白色念珠菌的挑战时，Ag NP 涂层可有效防止真菌生长，如酵母产生的乙醇所测量的，并且不会损害成纤维细胞。乙醇的产量从对照中的 43.2±25.02µmol/mL 降低到所有银处理中的 3.6µmol/mL。数据表明，用 Ag NPs 涂覆的硅酮假体材料在体外与成纤维细胞相容，并具有抗真菌特性。 © 2017 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，106B：1038-1051，2018。
