Salaie Ranj Nadhim, Besinis Alexandros, Tredwin Christopher, Handy Richard D
Oral and Maxillofacial Surgery Department, Faculty of Dentistry, Tishk International University, Iraq.
School of Engineering, Faculty of Science and Engineering, University of Plymouth, UK.
Toxicol Rep. 2024 Oct 18;13:101776. doi: 10.1016/j.toxrep.2024.101776. eCollection 2024 Dec.
the controlled release of silver as a biocide from Ag-coated medical implants is desirable. However, the biocompatibility of Ag leachates is poorly understood. This study investigated the toxicity of silver released from the silver plated titanium implants to human primary osteoblast cells; and the effect of cell culture medium on the silver speciation and bioavailability.
Ti6Al4V discs were coated with Ag nanoparticles (NPs), silver plus hydroxyapatite (HA) nanoparticles (Ag+nHA), or Ag NPs plus microparticles (Ag+mHA). Primary human osteoblast cells were exposed to the leachates from the various discs for up to 7 days.
the total Ag concentrations released as leachate from the silver-plated titanium discs were 0.7-1.6 mg L, regardless of treatment. Cumulative silver release over 7 days was approximately 3 mg L in all treatments. The concentration of total Ag in the cell homogenates from all the Ag-containing treatments was modest, ∼ 0.1 µg mg protein or less at day 7. Cells showed normal healthy morphology with no appreciable leak of LDH or ALP activity into the external media compared to the reference control. Similarly, there was no significant differences (Kruskal Wallis, > 0.05) in the LDH or ALP activity in the cell homogenate between treatments.
overall, there was a controlled release of Ag into the external media, but this remained biocompatible with no deleterious effects on the osteoblast cells, which means that the released silver to the peri-implant environment is not toxic making the coating potential for clinical use.
将银作为杀菌剂从涂银的医用植入物中进行控释是理想的。然而，人们对银浸出液的生物相容性了解甚少。本研究调查了镀银钛植入物释放的银对人原代成骨细胞的毒性；以及细胞培养基对银形态和生物利用度的影响。
在Ti6Al4V圆盘上涂覆银纳米颗粒（NPs）、银加羟基磷灰石（HA）纳米颗粒（Ag + nHA）或银纳米颗粒加微粒（Ag + mHA）。将原代人成骨细胞暴露于各种圆盘的浸出液中长达7天。
无论处理方式如何，镀银钛圆盘作为浸出液释放的总银浓度为0.7 - 1.6 mg/L。在所有处理中，7天内银的累积释放量约为3 mg/L。在第7天，所有含银处理的细胞匀浆中总银浓度适中，约为0.1 μg/mg蛋白质或更低。与参考对照相比，细胞呈现正常健康的形态，没有明显的乳酸脱氢酶（LDH）或碱性磷酸酶（ALP）活性泄漏到外部培养基中。同样，处理之间细胞匀浆中LDH或ALP活性没有显著差异（Kruskal Wallis检验，P > 0.05）。
总体而言，银向外部培养基中有控释放，但这仍具有生物相容性，对成骨细胞没有有害影响，这意味着释放到植入物周围环境中的银没有毒性，使得该涂层具有临床应用潜力。
