Veldhuijzen J P, Huisman A H, Vermeiden J P, Prahl-Andersen B
Connect Tissue Res. 1987;16(2):187-96. doi: 10.3109/03008208709002006.
Chick epiphyseal chondrocytes are isolated from 15 day old embryos and subsequently grown as small high density cultures (aggregates consisting of 2 X 10(5) cells), which can be handled individually. Histological evaluation showed a considerable increase in the dimensions of the nucleus and the cells 2-6 hours after initiating the growth of the chondrocytes by adding 10% serum to the culture medium. After 24 hours of culture the proportion of the matrix in the aggregate was increased from 0% to about 45%. In a series of experiments these aggregates were exposed to intermittent compressive force (ICF) with a peak value of 130 mbar above ambient and a frequency of 0.3 Hz. The nuclear and cell-dimensions of ICF-exposed chondrocytes were significantly larger than under control conditions when measured after 24 and 48 hours. Concomitantly, chondrocytes in ICF-exposed aggregates synthesized and deposited significantly more matrix in the aggregates as compared to controls. This model can be used to further study the direct effect of mechanical force on the synthesis and deposition of matrix components by cartilage cells in vitro.
鸡骨骺软骨细胞从15日龄胚胎中分离出来，随后以小的高密度培养物（由2×10⁵个细胞组成的聚集体）形式生长，这些聚集体可以单独处理。组织学评估显示，在向培养基中添加10%血清启动软骨细胞生长后2 - 6小时，细胞核和细胞的尺寸显著增加。培养24小时后，聚集体中基质的比例从0%增加到约45%。在一系列实验中，这些聚集体受到间歇性压缩力（ICF）作用，峰值比环境压力高130毫巴，频率为0.3赫兹。在24小时和48小时后测量时，暴露于ICF的软骨细胞的细胞核和细胞尺寸明显大于对照条件下的尺寸。同时，与对照组相比，暴露于ICF的聚集体中的软骨细胞在聚集体中合成和沉积的基质明显更多。该模型可用于进一步研究机械力对体外软骨细胞基质成分合成和沉积的直接影响。
