Buck Institute for Research on Aging, 8001 Redwood Blvd., Novato, CA 94945, USA.
Buck Institute for Research on Aging, 8001 Redwood Blvd., Novato, CA 94945, USA; Departments of Biochemistry and Physiology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore.
Biochim Biophys Acta Mol Basis Dis. 2018 Sep;1864(9 Pt A):2690-2696. doi: 10.1016/j.bbadis.2018.02.023. Epub 2018 Mar 8.
It has been nearly three decades since the budding yeast Saccharomyces cerevisiae became a significant model organism for aging research and it has emerged as both simple and powerful. The replicative aging assay, which interrogates the number of times a "mother" cell can divide and produce "daughters", has been a stalwart in these studies, and genetic approaches have led to the identification of hundreds of genes impacting lifespan. More recently, cell biological and biochemical approaches have been developed to determine how cellular processes become altered with age. Together, the tools are in place to develop a holistic view of aging in this single-celled organism. Here, we summarize the current state of understanding of yeast replicative aging with a focus on the recent studies that shed new light on how aging pathways interact to modulate lifespan in yeast.
自 budding yeast Saccharomyces cerevisiae 成为衰老研究的重要模式生物以来，已经将近三十年了，它已经变得既简单又强大。复制性衰老测定法，即探究“母”细胞可以分裂并产生“女儿”细胞的次数，一直是这些研究中的重要手段，遗传方法已经确定了数百个影响寿命的基因。最近，细胞生物学和生物化学方法也被开发出来，以确定随着年龄的增长，细胞过程如何发生改变。这些工具一起为在这个单细胞生物中发展出对衰老的整体认识奠定了基础。在这里，我们总结了目前对酵母复制性衰老的理解状况，重点介绍了最近的研究，这些研究揭示了衰老途径如何相互作用来调节酵母的寿命。
