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J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Apr 15;1083:57-62. doi: 10.1016/j.jchromb.2018.02.039. Epub 2018 Mar 2.
Acetyl coenzyme A is involved in several key metabolic pathways. Its concentration can vary considerably in response to physiological or pathological conditions making it a potentially valuable biomarker. However, little information about the measurement and concentration of acetyl CoA in human whole blood is found in the literature. The aim of this study was the development of an accurate method for the determination of acetyl CoA in human whole blood by LC-MS/MS. The method, involving extraction from whole blood by a rapid protein precipitation procedure was thoroughly validated: limit of quantitation was 3.91 ng mL. Accuracy and precision were calculated at five concentrations and were within ±15%. The average endogenous level of acetyl CoA in human whole blood was determined in 17 healthy individuals to be 220.9 ng mL (ranging from 124.0 to 308.0 ng mL). This represents, to our knowledge, the first report of acetyl CoA levels in human whole blood, and the first practical and reliable method for its determination.
乙酰辅酶 A 参与了几个关键的代谢途径。它的浓度在生理或病理条件下会有很大的变化，因此它是一种有潜在价值的生物标志物。然而，文献中关于人全血中乙酰辅酶 A 的测量和浓度的信息很少。本研究的目的是开发一种通过 LC-MS/MS 准确测定人全血中乙酰辅酶 A 的方法。该方法通过快速蛋白质沉淀程序从全血中提取，经过彻底验证：定量下限为 3.91ng/mL。在五个浓度下计算准确度和精密度，均在 ±15%范围内。在 17 名健康个体中测定人全血中乙酰辅酶 A 的平均内源性水平为 220.9ng/mL（范围为 124.0 至 308.0ng/mL）。据我们所知，这是首次报道人全血中的乙酰辅酶 A 水平，也是首次报道其实用可靠的测定方法。
