Lindqvist Dan Nybro, Pedersen Henrik Ærenlund, Rasmussen Lars Holm
Department of Technology, Metropolitan University College, Denmark.
Natural History Museum of Denmark, University of Copenhagen, Denmark.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Apr 1;1081-1082:126-130. doi: 10.1016/j.jchromb.2018.02.019. Epub 2018 Feb 18.
The dominant components in floral nectar is fructose, glucose and sucrose. The concentration and the ratio between the sugars are indicative for plant species and play an important part in the interplay between plants and pollinators. In this paper we present a novel HPLC-ELSD based analytical method for sugar characterization of nectar from orchids. Nectar was collected on Whatman No. 1 paper and preserved in the field by 70 v/v% ethanol. The analytical method had a linear range up to at least 3000 mg L for all 3 sugars with a precision of 1.5-1.7%. Correlation coefficients were 0.9999 to 1.0000. The LOD of all sugars were 5-7 mg L and the LOQ were 17-19 mg L. Field samples were stable for min. 7 weeks at -18 °C. The technique was applied to two species of Platanthera (Orchidaceae) in order to test whether species-related differences in sugar composition could be observed. No differences were found between the two species, which were sucrose-dominant (53.5-100%) though with high variation within species and between individual flowers.
花蜜中的主要成分是果糖、葡萄糖和蔗糖。糖的浓度和比例因植物种类而异，并且在植物与传粉者之间的相互作用中起着重要作用。在本文中，我们提出了一种基于高效液相色谱 - 蒸发光散射检测器（HPLC - ELSD）的新颖分析方法，用于表征兰花花蜜中的糖分。花蜜收集在沃特曼1号滤纸上，并在野外通过70%（v/v）乙醇保存。该分析方法对所有3种糖的线性范围至少达到3000 mg/L，精密度为1.5 - 1.7%。相关系数为0.9999至1.0000。所有糖的检测限为5 - 7 mg/L，定量限为17 - 19 mg/L。野外样品在 - 18°C下至少7周内保持稳定。该技术应用于两种舌唇兰属（兰科）植物，以测试是否能观察到与物种相关的糖组成差异。两种植物之间未发现差异，它们均以蔗糖为主（53.5 - 100%），不过物种内部和单个花朵之间存在很大差异。
