Guffa Basem, Nedić Nebojša M, Dabić Zagorac Dragana Č, Tosti Tomislav B, Gašić Uroš M, Natić Maja M, Fotirić Akšić Milica M
Faculty of Agriculture, University of Belgrade, Nemanjina 6, 11080, Zemun, Serbia.
Innovation Center, Faculty of Chemistry Ltd, University of Belgrade, Studentski trg 12-16, 11000, Belgrade, Serbia.
Chem Biodivers. 2017 Sep;14(9). doi: 10.1002/cbdv.201700061.
'Oblačinska' sour cherry, an autochthonous cultivar, is the most planted cultivar in Serbian orchards. Since fruit trees in temperate zone reward insects by producing nectar which 'quality' affects the efficiency of insect pollination, the aim of this study was analyzing of sugars and polyphenolics in floral nectar of 16 'Oblačinska' sour cherry clones with different yielding potential. The contents of sugars and sugar alcohols were analyzed by ion chromatography, while polyphenolic profile was established using liquid chromatography/mass spectrometry technique. Fourteen sugars and six sugar alcohols were detected in nectar samples and the most abundant were fructose, glucose, and sucrose. Eleven polyphenols were quantified using available standards, while another 17 were identified according to their exact masses and characteristic fragmentations. Among quantified polyphenols, rutin, naringenin, and chrysin were the most abundant in nectar. Principal component analysis showed that some polyphenol components (naringin, naringenin, and rutin) together with sugars had high impact of spatial distribution of nectar samples on score plot.
“奥布拉钦斯卡”酸樱桃是一个本土品种，是塞尔维亚果园中种植最多的品种。由于温带地区的果树通过分泌花蜜来吸引昆虫，而花蜜的“质量”会影响昆虫授粉的效率，因此本研究的目的是分析16个具有不同产量潜力的“奥布拉钦斯卡”酸樱桃克隆品种花蜜中的糖分和多酚类物质。通过离子色谱法分析糖和糖醇的含量，同时使用液相色谱/质谱技术确定多酚类物质的谱图。在花蜜样品中检测到14种糖和6种糖醇，其中含量最高的是果糖、葡萄糖和蔗糖。使用现有标准对11种多酚进行了定量分析，另外17种则根据其精确质量和特征性裂解进行了鉴定。在定量分析的多酚中，芦丁、柚皮素和白杨素在花蜜中的含量最高。主成分分析表明，一些多酚成分（柚皮苷、柚皮素和芦丁）与糖分一起对花蜜样品在得分图上的空间分布有很大影响。
