RWTH Aachen University, Institute for Environmental Research, Department of Ecosystem Analysis, Aachen, Germany.
RWTH Aachen University, Institute for Environmental Research, Environmental Biology and Chemodynamics, Aachen, Germany; Chongqing University, College of Resources and Environmental Science, Chongqing, China; Nanjing University, State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing, China.
Sci Total Environ. 2018 Aug 1;631-632:216-222. doi: 10.1016/j.scitotenv.2018.03.028. Epub 2018 Mar 16.
The ecotoxicity of two biofuel candidates (1‑octanol and 2‑butanone) was investigated by an integrative test strategy using three bioassays: the acute immobilisation test with water flea (D. magna), the fish embryo acute toxicity test with zebrafish (Danio rerio) and the in vitro micronucleus assay with Chinese hamster (Cricetulus griseus) V79 cells. The median effective concentration (EC) values were 14.9±0.66mgL for 1‑octanol, and 2152.1±44.6mgL for 2‑butanone in the D. magna test. Both 1‑octanol and 2‑butanone caused teratogenic and lethal effects on zebrafish embryos, while exposure to 1‑octanol significantly induced these effects at concentrations ≥2.0mgL. These results indicate that 1‑octanol exert much higher ecotoxicity than 2‑butanone to D. magna and zebrafish embryos. Moreover, both 1‑octanol and 2‑butanone did not cause significant genotoxic effects, while their metabolites significantly induced micronuclei in V79 cells. The present study proposed an integrative test approach to evaluate the potential ecotoxicity of biofuels using simple, quick and inexpensive bioassays.
采用三种生物测定方法（水蚤急性固定试验、斑马鱼胚胎急性毒性试验和中国仓鼠肺成纤维细胞（V79）微核试验），用综合测试策略研究了两种生物燃料候选物（1-辛醇和 2-丁酮）的生态毒性。1-辛醇在水蚤试验中的中值有效浓度（EC）值为 14.9±0.66mgL，2-丁酮的 EC 值为 2152.1±44.6mgL。1-辛醇和 2-丁酮均对斑马鱼胚胎产生致畸和致死作用，而 1-辛醇在浓度≥2.0mgL 时显著诱导这些作用。这些结果表明，1-辛醇对水蚤和斑马鱼胚胎的生态毒性比 2-丁酮高得多。此外，1-辛醇和 2-丁酮均未引起明显的遗传毒性作用，而它们的代谢物在 V79 细胞中显著诱导了微核。本研究提出了一种综合测试方法，使用简单、快速和廉价的生物测定法来评估生物燃料的潜在生态毒性。
