Christie Andrew E, Pascual Micah G, Yu Andy
Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.
Békésy Laboratory of Neurobiology, Pacific Biosciences Research Center, School of Ocean and Earth Science and Technology, University of Hawaii at Manoa, 1993 East-West Road, Honolulu, HI 96822, USA.
Mar Genomics. 2018 Jun;39:45-63. doi: 10.1016/j.margen.2018.01.005. Epub 2018 Mar 8.
Peptides are the largest/most diverse class of molecules used by animals for chemical communication, and with their cognate receptors, are key players in modulating physiological/behavioral control systems, including those involved in adaptation to environmental change. Crustaceans have long served as models for investigating peptidergic control of physiology/behavior, and members of Notostraca, an ancient branchiopod order, have recently been proposed as models for investigating the genetic/physiological underpinnings of ecoresponsiveness; nothing is currently known about the genes/proteins underlying peptidergic signaling in any member of this crustacean taxon. Transcriptome mining is a powerful tool for peptidome prediction in crustaceans, and all large-scale discovery of crustacean peptide receptors has been achieved via transcriptomics. Here, in silico transcriptome mining was used to elucidate the peptidergic signaling systems of the tadpole shrimp Triops newberryi, a member of Notostraca. Transcripts encoding putative precursor proteins and/or receptors for 28 peptide families were identified within the T. newberryi dataset. The deduced precursor proteins included those for allatostatin A, allatostatin B, allatostatin C, allatotropin, bursicon, CCHamide, crustacean cardioactive peptide, crustacean hyperglycemic hormone, diuretic hormone 44, ecdysis-triggering hormone, eclosion hormone, elevenin, FMRFamide-like peptide, glycoprotein hormone, GSEFLamide, inotocin, insulin-like peptide, neuroparsin, neuropeptide F, orcokinin/orcomyotropin, proctolin, pyrokinin/periviscerokinin, SIFamide, sulfakinin and tachykinin-related peptide; 117 distinct mature peptides were predicted from the collective set of deduced pre/preprohormones. Transcripts encoding putative receptors for most of the abovementioned peptide groups were also identified from the T. newberryi assembly, as were those for several families for which no precursors were found, i.e., corazonin, RYamide and short neuropeptide F. This is the first description of a peptidome and peptide receptors from any member of the Notostraca, and as such, provide a foundation for beginning to investigate the roles played by peptidergic signaling systems in T. newberryi and other notostracans, including how they may contribute to modulating organism-environment interactions.
肽是动物用于化学通讯的最大/最多样化的分子类别，并且与它们的同源受体一起，是调节生理/行为控制系统的关键参与者，包括那些参与适应环境变化的系统。甲壳类动物长期以来一直是研究肽能对生理/行为控制的模型，而背甲目（一种古老的鳃足纲目）的成员最近被提议作为研究生态响应的遗传/生理基础的模型；目前对于这个甲壳类分类群中任何成员的肽能信号传导所涉及的基因/蛋白质一无所知。转录组挖掘是预测甲壳类动物肽组的有力工具，并且所有大规模发现的甲壳类动物肽受体都是通过转录组学实现的。在这里，利用计算机模拟转录组挖掘来阐明蝌蚪虾（Triops newberryi，背甲目的成员）的肽能信号系统。在Triops newberryi数据集中鉴定出了编码28个肽家族的假定前体蛋白和/或受体的转录本。推导的前体蛋白包括抑咽侧体素A、抑咽侧体素B、抑咽侧体素C、促咽侧体素、羽化激素、CCH酰胺、甲壳类心脏活性肽、甲壳类高血糖激素、利尿激素44、蜕皮触发激素、羽化激素、十一肽、FMRF酰胺样肽、糖蛋白激素、GSEFL酰胺、肌肽、胰岛素样肽、神经垂体素、神经肽F、orcokinin/orcomyotropin、促肠肌肽、焦激肽/内脏周激肽、SIF酰胺、磺激肽和速激肽相关肽的前体蛋白；从推导的前/前原激素的集合中预测出了117种不同的成熟肽。从Triops newberryi组装体中还鉴定出了编码上述大多数肽组假定受体的转录本，以及几个未发现前体的家族（即促心肽、RY酰胺和短神经肽F）的转录本。这是对背甲目任何成员的肽组和肽受体的首次描述，因此为开始研究肽能信号系统在Triops newberryi和其他背甲类动物中所起的作用奠定了基础，包括它们如何可能有助于调节生物体与环境的相互作用。
