山羊流产疫情中弓形虫克隆谱系II型（基因型#1）的首次描述。
First description of clonal lineage type II (genotype #1) of Toxoplasma gondii in abortion outbreak in goats.
作者信息
de Oliveira Júnior Mário Baltazar, de Almeida Jonatas Campos, de Melo Renata Pimentel Bandeira, de Barros Luiz Daniel, Garcia João Luis, Andrade Müller Ribeiro, Porto Wagnner José Nascimento, Regidor-Cerrillo Javier, Ortega-Mora Luis Miguel, Oliveira Andréa Alice da Fonseca, Mota Rinaldo Aparecido
机构信息
Department of Veterinary Medicine, Laboratory of Infectious-Contagious Diseases of Domestic Animals, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, 52171-900, Recife, PE, Brazil.
Department of Veterinary Medicine, Laboratory of Infectious-Contagious Diseases of Domestic Animals, Universidade Federal Rural de Pernambuco, Rua Dom Manoel de Medeiros, 52171-900, Recife, PE, Brazil.
出版信息
Exp Parasitol. 2018 May;188:21-25. doi: 10.1016/j.exppara.2018.03.008. Epub 2018 Mar 8.
The purpose of this study was to perform genotypic characterization and to evaluate the virulence of Toxoplasma gondii obtained from aborted fetuses in an abortion outbreak in goats from northeastern Brazil. Brain samples from 32 fetuses were submitted to mouse bioassay for T. gondii isolation. Two isolates were obtained and subjected to genotypic characterization. Isolate virulence was evaluated using murine model in different doses (from 10 to 10 tachyzoites/mL). In genotyping, both isolates were classified as clonal lineage type II (genotype #1 ToxoDB) and showed to be virulent for mice. This is the first description of genotype #1 in cases of goat abortion, showing the circulation of virulent T. gondii isolate producing reproductive disorders in pregnant goat.
本研究的目的是对从巴西东北部山羊流产疫情中流产胎儿分离得到的刚地弓形虫进行基因型鉴定，并评估其毒力。从32只胎儿的脑样本中进行小鼠生物测定以分离刚地弓形虫。获得了两株分离株并进行基因型鉴定。使用小鼠模型以不同剂量（从10到10速殖子/毫升）评估分离株的毒力。在基因分型中，两株分离株均被归类为克隆谱系II型（基因型#1 ToxoDB），并对小鼠具有毒力。这是山羊流产病例中基因型#1的首次描述，表明产毒的刚地弓形虫分离株在怀孕山羊中传播导致生殖障碍。
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