Department of Ocean Technology, College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng, 224051, China.
Department of Ocean Technology, College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng, 224051, China; Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University, Dalian, 116023, China.
Fish Shellfish Immunol. 2018 May;76:266-271. doi: 10.1016/j.fsi.2018.03.013. Epub 2018 Mar 9.
Sea cucumber, Apostichopus japonicus, is one of the most important holothurian species cultured in China. Severe evisceration induced by various natural and artificial factors commonly occurs during transport and culture of A. japonicus. Evisceration causes higher mortality and lower yield. Along with the visceral regeneration process, sea cucumbers also regenerate coelomocytes in order to recover immune function. In this study, evisceration of A. japonicus was induced by intracoelomic injection of 0.35 M KCl. Regeneration of coelomocytes was investigated by time course cell counting as well as detection of DNA replication by the EdU labeling technique. Coelomic fluid volume was restored to the pre-evisceration level within 2 h after evisceration. Total coelomocyte count (TCC) reached a peak at 6 h post-evisceration, followed decreased and then increased with a slight fluctuation, restored to the pre-evisceration level at 35 d post-evisceration. The change in different subtypes of coelomocytes was consistent with that of total coelomocytes. However, there were some variations in the regeneration of coelomocyte subtypes. At the end of the study, only the counts of amoebocytes and morula cells recovered to the pre-evisceration level. DNA replication assay showed EdU-positive cells accounted for 9.5% before evisceration and 4.7% at 6 h post-evisceration. However, the percentage of EdU-positive cells significantly increased, reaching 18.6% at 3 d after evisceration, then declined. Therefore, we analyzed the observed increase in coelomocytes at 6 h post-evisceration, which may be due to coelomocyte migration from the water-vascular system into the coelom rather than de novo cell proliferation.
海参是中国最重要的养殖棘皮动物之一，海地瓜。在运输和养殖过程中，各种自然和人为因素都会导致海地瓜严重的内脏排空。内脏排空会导致更高的死亡率和更低的产量。随着内脏再生过程的进行，海参还会再生体腔细胞以恢复免疫功能。在这项研究中，通过腔内注射 0.35 M KCl 诱导海地瓜内脏排空。通过时间进程细胞计数以及 EdU 标记技术检测 DNA 复制来研究体腔细胞的再生。内脏排空后 2 小时内，体腔液体积恢复到排空前水平。总体腔细胞计数（TCC）在排空后 6 小时达到峰值，随后下降，然后略有波动，在排空后 35 天恢复到排空前水平。不同亚型体腔细胞的变化与总体腔细胞的变化一致。然而，体腔细胞亚型的再生存在一些差异。在研究结束时，只有变形细胞和原肠胚细胞的计数恢复到排空前水平。DNA 复制分析表明，排空前 EdU 阳性细胞占 9.5%，排空后 6 小时占 4.7%。然而，EdU 阳性细胞的百分比显著增加，在排空后 3 天达到 18.6%，然后下降。因此，我们分析了排空后 6 小时观察到的体腔细胞增加，这可能是由于体腔细胞从水血管系统迁移到体腔，而不是新的细胞增殖。
