Milošević Verica Lj, Severs Walter B, Ristić Nataša M, Manojlović-Stojanoski Milica N, Popovska-Perčinić Florina V, Šošić-Jurjević Branka T, Pendovski Lazo B, Trifunović Svetlana L, Miler Marko Š, Ajdžanović Vladimir Z
Department of Cytology, Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia.
Department of Pharmacology, College of Medicine, Pennsylvania State University, Hershey, Pa., USA.
Histol Histopathol. 2018 Aug;33(8):843-857. doi: 10.14670/HH-11-984. Epub 2018 Mar 12.
Genistein (G) and related soy phytoestrogens have been studied for potential usefulness in different chronic diseases, and may ameliorate signs of aging. They have a profound influence on the hypothalamo-pituitary-adrenal (HPA) axis. The present study utilized the rat model of mild andropause to thoroughly evaluate the effects of G and soy extract on the adrenal gland and related blood hormones. Adult male rats were orchidectomized (Orx) or sham operated (SO). Orx rats received daily subcutaneous injections for 3 weeks of solvent, or G (Orx+G, 30 mg/kg), or commercial soy extract (Orx+Soy, 30 mg/kg). Adrenal glands and blood were harvested at the end of the treatment for hormone analyses, histology and design-based stereology. Compared to SO rats Orx evoked significant (P<0.05) changes including: the replicating cell number in the 3 adrenocortical zones; vascularity and cortical volume and blood levels of adrenocorticotropic hormone (ACTH), aldosterone and dehydroepiandrosterone (DHEA). When comparing Orx vs. Orx+G groups the following significant (P<0.05) changes were observed: a further increase in number of replicating cells in zonas glomerulosa and reticularis, vasculature network presence, cortical and zona reticularis volumes, ACTH and corticosterone concentrations, and lower DHEA levels. Comparing Orx vs. Orx+Soy resulted in elevated (P<0.05) ACTH and corticosterone levels. Structural integrity of the adrenal gland was unchanged vs. SO rats. Overall, G and soy extract treatments resulted in proliferative activity and/or vasculature support in the adrenal cortex. The data and current literature support the impression of a beneficial effect of soy components on the homeostatic response to stress.
染料木黄酮（G）及相关大豆植物雌激素已被研究其在不同慢性疾病中的潜在用途，并且可能改善衰老迹象。它们对下丘脑-垂体-肾上腺（HPA）轴有深远影响。本研究利用轻度雄性更年期大鼠模型，全面评估G和大豆提取物对肾上腺及相关血液激素的影响。成年雄性大鼠接受去势手术（Orx）或假手术（SO）。去势大鼠连续3周每天皮下注射溶剂、G（Orx+G，30毫克/千克）或市售大豆提取物（Orx+Soy，30毫克/千克）。治疗结束时采集肾上腺和血液进行激素分析、组织学和基于设计的体视学分析。与假手术大鼠相比，去势引起显著（P<0.05）变化，包括：三个肾上腺皮质区的复制细胞数量；血管生成、皮质体积以及促肾上腺皮质激素（ACTH）、醛固酮和脱氢表雄酮（DHEA）的血液水平。比较去势组与去势+G组时，观察到以下显著（P<0.05）变化：球状带和网状带复制细胞数量进一步增加、存在血管网络、皮质和网状带体积、ACTH和皮质酮浓度升高，以及DHEA水平降低。比较去势组与去势+大豆组导致ACTH和皮质酮水平升高（P<0.05）。与假手术大鼠相比，肾上腺的结构完整性未改变。总体而言，G和大豆提取物治疗导致肾上腺皮质的增殖活性和/或血管支持。这些数据和当前文献支持大豆成分对应激稳态反应具有有益作用的观点。
