Department of Cytology, University of Belgrade, Institute for Biological Research "Siniša Stanković,", Belgrade, 11060, Serbia.
Anat Rec (Hoboken). 2018 Aug;301(8):1416-1425. doi: 10.1002/ar.23809. Epub 2018 Mar 30.
The aim of the study was to examine the potential of the principal soy isoflavones, genistein and daidzein, or isoflavone rich soy extract to recover pituitary castration cells in orchidectomized adult male rats in comparison with the effects of estradiol. Two weeks post orchidectomy (Orx), animals received estradiol-dipropionate, genistein, daidzein or soy extract subcutaneously for 3 weeks. Control sham-operated (So) and Orx rats received just the vehicle. Changes in the volumes of pars distalis, of individual follicle-stimulating hormone (FSH) and luteinizing hormone (LH) containing cells, their volume, numerical density and number were determined by unbiased design-based stereology. The intracellular content of βFSH and βLH was estimated by relative intensity of fluorescence (RIF). Orchidectomy increased all examined stereological parameters and RIF. Compared to Orx, estradiol increased the volume of pars distalis, but reversed RIF and all morphometric parameters of gonadotropes to the level of So rats, except their number. Treatments with purified isoflavones and soy extract decreased RIF to the control So level, expressing an estradiol-like effect. However, the histological appearance and morphometrical features of gonadotropes did not follow this pattern. Genistein increased the volume of pars distalis, decreased the volume density of LH-labeled cells and raised the number of gonadotropes. Daidzein decreased the cell volume of gonadotropic cells but increased their number and numerical density. Soy extract induced an increase in number and numerical density of FSH-containing cells. Therefore, it can be concluded that soy phytoestrogens do not fully reverse the Orx-induced changes in pituitary castration cells. Anat Rec, 2018. © 2018 Wiley Periodicals, Inc.
本研究旨在探讨主要大豆异黄酮(genistein 和 daidzein)或富含异黄酮的大豆提取物恢复去势成年雄性大鼠垂体细胞的潜力，并与雌二醇的作用进行比较。去势后 2 周（Orx），动物接受雌二醇二丙酸酯、genistein、daidzein 或大豆提取物皮下注射 3 周。对照假手术（So）和 Orx 大鼠仅接受载体。通过无偏设计的基于体视学方法确定脑垂体远侧部的体积、单个促卵泡激素（FSH）和促黄体生成激素（LH）的体积、细胞数密度和数量的变化。通过相对荧光强度（RIF）估计βFSH 和βLH 的细胞内含量。去势增加了所有检查的体视学参数和 RIF。与 Orx 相比，雌二醇增加了脑垂体远侧部的体积，但将促性腺激素细胞的 RIF 和所有形态计量学参数逆转至 So 大鼠的水平，除了它们的数量。用纯化的异黄酮和大豆提取物处理降低了 RIF 至对照 So 水平，表现出类似雌激素的作用。然而，促性腺激素细胞的组织学外观和形态计量学特征并未遵循这种模式。Genistein 增加了脑垂体远侧部的体积，降低了 LH 标记细胞的体积密度，并增加了促性腺激素的数量。Daidzein 降低了促性腺细胞的细胞体积，但增加了它们的数量和细胞数密度。大豆提取物诱导 FSH 阳性细胞的数量和细胞数密度增加。因此，可以得出结论，大豆植物雌激素不能完全逆转去势引起的垂体细胞变化。解剖学记录，2018. © 2018 Wiley Periodicals，Inc.
