Yamaguchi Y
J Chromatogr. 1979 Jul 21;163(3):253-8. doi: 10.1016/s0378-4347(00)81412-7.
The fractional assay is described of 3 beta-hydroxysteroids in various patients by thin-layer chromatography and color development using an enzyme that reacts specifically with some 3 beta-hydroxysteroids. Together with dehydroepiandrosterone, androst-5-ene-3 beta,17 beta-diol and 16 alpha-hydroxydehydroepiandrosterone can be detected, but their concentrations differ with each disease. An unknown fraction, a more polar 3 beta-hydroxysteroid than 16 alpha-hydroxydehydroepiandrosterone, is also detected in moderate amounts in patients with adrenal tumor (18.3 mg/day), 21-hydroxylase deficiency (3.2 to 1.2 mg/day), and Cushing's syndrome (0.9-2.3 mg/day, as pregn-5-ene-triol).
采用薄层色谱法及显色法，利用一种能与某些3β-羟基类固醇特异性反应的酶，对不同患者体内的3种β-羟基类固醇进行了定量分析。除脱氢表雄酮外，还可检测到雄甾-5-烯-3β,17β-二醇和16α-羟基脱氢表雄酮，但其浓度因疾病而异。在肾上腺肿瘤患者（18.3毫克/天）、21-羟化酶缺乏症患者（3.2至1.2毫克/天）及库欣综合征患者（以孕-5-烯三醇计为0.9 - 2.3毫克/天）体内，还检测到了一种未知组分，这是一种比16α-羟基脱氢表雄酮极性更强的3β-羟基类固醇，含量适中。
