Yamaguchi Y
Clin Chem. 1980 Mar;26(3):491-3.
I describe the use of 3 alpha-hydroxysteroid dehydrogenase (EC 1.1.1.50) for enzymic color development of 3 alpha-hydroxysteroids. Urinary 3 alpha-hydroxysteroids are converted to 3-ketosteroids by the enzyme, and the NADH formed is reacted with 2-p-iodophenyl-3-p-nitrophenyl-5-phenyl-tetrazolium chloride and "diaphorase" to form the colored formazan. The dye so formed on a thin layer chromatographic plate is measured by densitometoric scanning. I illustrate use of this method by showing results for urinary excretion of 3 alpha-hydroxysteroids by some various sorts of patients.
我描述了使用3α-羟基类固醇脱氢酶（EC 1.1.1.50）对3α-羟基类固醇进行酶促显色的方法。尿中的3α-羟基类固醇被该酶转化为3-酮类固醇，生成的NADH与2-对碘苯基-3-对硝基苯基-5-苯基氯化四氮唑和“黄递酶”反应形成有色的甲臜。在薄层色谱板上形成的染料通过光密度扫描进行测量。我通过展示各类患者尿中3α-羟基类固醇排泄的结果来说明该方法的应用。
