Matsson L, Theilade J, Attström R
J Clin Periodontol. 1979 Dec;6(6):425-36. doi: 10.1111/j.1600-051x.1979.tb01941.x.
A previous study demonstrated structural differences in the junctional epithelium between juvenile and adult dogs. In juveniles the junctional epithelium showed some resemblances to the oral gingival epithelium, and a cuticular structure at the dento-gingival junction was of particular interest. The oral gingival epithelium is considered to be less permeable than the junctional epithelium. As cytoplasmic filaments are held to be the main component in the process of keratinization, and to have a stabilizing influence on the cells and tissues, the present investigation was designed to study the relative amounts of cytoplasmic filaments in the junctional and the oral epithelia of beagle dogs during juvenile and adult stages. In addition, the ultrastructure of the dento-gingival junction was characterized. Six beagle dogs were used. The material consisted of gingival biopsies sampled when the dogs were 3 and 12 months old, respectively. On these occasions the gingiva was in excellent health. The biopsies were prepared for electron microscopic analysis and three randomly selected fields were recorded photographically from each of the following epithelial strata: basal and granular cell layers of the oral epithelium and basal and superficial cell layers of the junctional epithelium. Morphometric analysis was performed in order to estimate the density of cytoplasmic filaments of the cells in these epithelial strata. The amount of cytoplasmic filaments was considerably lower in the cells of the junctional epithelium than in those of the oral epithelium. In the oral epithelium the amount increased from basal towards superficial cells, whereas no such increase was seen in the junctional epithelium. The pattern was the same in the juvenile and the adult stage. At the dento-gingival junction all dogs had a thick, laminated layer of dental cuticle material in the juvenile stage. In the adult stage a similar structure was seen only infrequently, and it never attained the thickness observed in the juvenile stage.
先前的一项研究表明，幼年犬和成年犬的结合上皮存在结构差异。在幼年犬中，结合上皮与口腔牙龈上皮有一些相似之处，牙-龈交界处的角质层结构尤其令人感兴趣。口腔牙龈上皮被认为比结合上皮的通透性更低。由于细胞质细丝被认为是角质化过程中的主要成分，并且对细胞和组织有稳定作用，因此本研究旨在研究幼年和成年比格犬结合上皮和口腔上皮中细胞质细丝的相对含量。此外，还对牙-龈交界处的超微结构进行了表征。使用了六只比格犬。材料包括分别在犬3个月和12个月大时采集的牙龈活检样本。在这些时候，牙龈健康状况良好。活检样本被制备用于电子显微镜分析，并从以下每个上皮层中随机选择三个视野进行拍照记录：口腔上皮的基底细胞层和颗粒细胞层以及结合上皮的基底细胞层和表层细胞层。进行形态计量分析以估计这些上皮层中细胞的细胞质细丝密度。结合上皮细胞中的细胞质细丝数量明显低于口腔上皮细胞中的。在口腔上皮中，数量从基底细胞向表层细胞增加，而在结合上皮中未见这种增加。幼年和成年阶段的模式相同。在牙-龈交界处，所有犬在幼年阶段都有一层厚厚的、分层的牙釉质材料层。在成年阶段，仅偶尔可见类似结构，且其厚度从未达到幼年阶段观察到的厚度。
