Steflik D E, McKinney R V, Koth D L, Singh B B
Scan Electron Microsc. 1984(Pt 2):547-55.
The interface between oral tissues and ceramic endosteal dental implants was investigated in 36 experimental cases in dog jaws. Conventional electron microscopic modalities showed those tissues adjacent to the implants to be viable with no adverse cellular response to the biomaterial. A procedure to retrieve additional ultrastructural data from previously processed biological material was developed to expand upon these data. This included surface-etching with oxygen plasma of routinely processed electron microscopy specimens and formalin fixed histological specimens for scanning electron microscopy (SEM) study; and, cryofracturing of similar histological specimens for additional transmission electron microscopic (TEM) analysis. Results from these alternative protocols supported our observations from conventional studies of the direct association of bone and epithelia to the implant. In addition, they demonstrated a unique portrayal of the epithelial maturation patterns. Further, this alternative TEM method showed excellent retention of cellular integrity and demonstrated a hemidesmosomal-external basal lamina attachment apparatus at the junctional epithelial-implant interface. This latter composite attachment structure was not retained in our previous conventional microdissection procedures. Thus, the interface between oral tissues and the single crystal sapphire endosteal dental implant could be elucidated by these protocols.
在36例犬颌骨实验病例中，对口腔组织与陶瓷骨内牙种植体之间的界面进行了研究。传统电子显微镜检查方式显示，与种植体相邻的组织具有活力，对生物材料没有不良细胞反应。为了扩展这些数据，开发了一种从先前处理过的生物材料中获取更多超微结构数据的方法。这包括对常规处理的电子显微镜标本和福尔马林固定的组织学标本进行氧等离子体表面蚀刻，用于扫描电子显微镜（SEM）研究；以及对类似的组织学标本进行冷冻断裂，用于额外的透射电子显微镜（TEM）分析。这些替代方案的结果支持了我们从骨和上皮与种植体直接关联的常规研究中的观察结果。此外，它们展示了上皮成熟模式的独特描绘。此外，这种替代的TEM方法显示细胞完整性保留良好，并在结合上皮 - 种植体界面处展示了半桥粒 - 外部基膜附着装置。后一种复合附着结构在我们之前的传统显微解剖程序中并未保留。因此，通过这些方案可以阐明口腔组织与单晶蓝宝石骨内牙种植体之间的界面。
