抑制脂多糖（LPS）诱导的 Khaya grandifoliola（C.D.C.）树皮、Cryptolepis sanguinolenta（Lindl.）Schltr 和 Cymbopogon citratus Stapf 叶的多糖部分对原代 264.7 巨噬细胞和 U87 神经胶质瘤细胞的神经炎症反应。
Inhibition of lipopolysaccharide (LPS)-induced neuroinflammatory response by polysaccharide fractions of Khaya grandifoliola (C.D.C.) stem bark, Cryptolepis sanguinolenta (Lindl.) Schltr and Cymbopogon citratus Stapf leaves in raw 264.7 macrophages and U87 glioblastoma cells.
机构信息
Laboratory of Phytoprotection and Valorisation of Plant Resources, The Biotechnology Centre, University of Yaounde I, P.O. Box 3851, Messa-Yaounde, Yaounde, Cameroon.
Department of Biochemistry, University of Yaounde I, P.O. Box 812, Yaounde, Cameroon.
出版信息
BMC Complement Altern Med. 2018 Mar 12;18(1):86. doi: 10.1186/s12906-018-2156-2.
BACKGROUND
Khaya grandifoliola (C.D.C.) stem bark, Cymbopogon citratus (Stapf) and Cryptolepis sanguinolenta (Lindl.) Schltr leaves are used in Cameroonian traditional medicine for the treatment of inflammatory diseases. Several studies have been performed on the biological activities of secondary metabolites extracted from these plants. However, to the best of our knowledge, the anti-neuro inflammatory and protective roles of the polysaccharides of these three plants have not yet been elucidated. This study aimed at investigating potential use of K. grandifoliola, C. sanguinolenta and C. citratus polysaccharides in the prevention of chronic inflammation.
METHODS
Firstly, the composition of polysaccharide fractions isolated from K. grandifoliola stem bark (KGF), C. sanguinolenta (CSF) and C. citratus (CCF) leaves was assessed. Secondly, the cytotoxicity was evaluated on Raw 264.7 macrophages and U87-MG glioblastoma cell lines by the MTT assay. This was followed by the in vitro evaluation of the ability of KGF, CSF and CCF to inhibit lipopolysaccharides (LPS) induced overproduction of various pro-inflammatory mediators (NO, ROS and IL1β, TNFα, IL6, NF-kB cytokines). This was done in Raw 264.7 and U87-MG cells. Finally, the in vitro protective effect of KGF, CSF and CCF against LPS-induced toxicity in the U87-MG cells was evaluated.
RESULTS
CCF was shown to mostly contain sugar and no polyphenol while KGP and CSP contained very few amounts of these metabolites (≤ 2%). The three polysaccharide fractions were non-toxic up to 100 μg.mL. All the polysaccharides at 10 μg/mL inhibited NO production, but only KGF and CCF at 12.5 μg/mL down-regulated LPS-induced ROS overproduction. Finally, 100 μg/mL LPS reduced 50% of U87 cell viability, and pre-treatment with the three polysaccharides significantly increased the proliferation.
CONCLUSION
These results suggest that the polysaccharides of K. grandifoliola, C. citratus and C. sanguinolenta could be beneficial in preventing/treating neurodegenerative diseases in which neuroinflammation is part of the pathophysiology.
背景
喀雅大戟（C.D.C.）树皮、香茅（Stapf）和血叶兰（Lindl.）Schltr 叶子在喀麦隆传统医学中用于治疗炎症性疾病。已经对这些植物中提取的次生代谢物的生物活性进行了多项研究。然而，据我们所知，这些植物多糖的抗神经炎症和保护作用尚未阐明。本研究旨在探讨喀雅大戟、血叶兰和香茅多糖在预防慢性炎症中的潜在用途。
方法
首先，评估了从喀雅大戟茎皮（KGF）、血叶兰（CSF）和香茅（CCF）叶子中分离的多糖级分的组成。其次，通过 MTT 测定法在 Raw 264.7 巨噬细胞和 U87-MG 神经胶质瘤细胞系上评估细胞毒性。接着，评估 KGF、CSF 和 CCF 抑制脂多糖（LPS）诱导的各种促炎介质（NO、ROS 和 IL1β、TNFα、IL6、NF-kB 细胞因子）过度产生的体外能力。在 Raw 264.7 和 U87-MG 细胞中进行了这项研究。最后，评估了 KGF、CSF 和 CCF 在 LPS 诱导的 U87-MG 细胞毒性中的体外保护作用。
结果
CCF 主要含有糖而不含多酚，而 KGP 和 CSP 则含有非常少量的这些代谢物（≤2%）。三种多糖级分的毒性均在 100μg.mL 以下。所有多糖在 10μg/mL 时均抑制 NO 产生，但仅 KGF 和 CCF 在 12.5μg/mL 时下调 LPS 诱导的 ROS 过度产生。最后，100μg/mL LPS 使 U87 细胞活力降低 50%，而三种多糖的预处理可显著增加增殖。
结论
这些结果表明，喀雅大戟、香茅和血叶兰的多糖可能有益于预防/治疗神经炎症是其病理生理学一部分的神经退行性疾病。
Zotero 插件