Kleveland P M, Waldum H L, Petersen H, Sandberg P
Scand J Gastroenterol. 1987 Mar;22(2):165-73. doi: 10.3109/00365528708991875.
The gastric pepsin output by the totally isolated, vagally denervated, and both luminally and vascularly perfused rat stomach was studied. Basal release of pepsin was 3% of total gastric mucosal stores per hour. Topical (luminal) administration of 0.001 M HCl was without effect. Graded concentrations of pentagastrin, gastrin, histamine, and impromidine in the vascular perfusate were tested. Pentagastrin induced only a slight increase in pepsin release (15% of total content per hour). Gastrin had a more pronounced effect and elicited a significant increase in the pepsin output, reaching a maximum of 28% of total pepsin content per hour. The CD50 of gastrin was 0.061 nM. Histamine and impromidine likewise produced significant increases in the pepsin secretion (maximum, 40% and 35% of total stores per hour, respectively). When the CD50 of histamine (0.718 microM) was compared with that of impromidine (0.011 microM), impromidine on molar basis had 65 times higher potency than histamine as a pepsin secretagogue. When the pepsin and acid responses during gastrin, histamine, and impromidine stimulation were compared, the pepsinogen secretion was shifted to the left and thus was more sensitive to stimulation than the acid secretion.
对完全分离、迷走神经切断且经腔内和血管灌注的大鼠胃的胃蛋白酶分泌进行了研究。胃蛋白酶的基础释放量为每小时胃黏膜总储存量的3%。局部（腔内）给予0.001 M盐酸无作用。对血管灌注液中不同浓度的五肽胃泌素、胃泌素、组胺和英普咪定进行了测试。五肽胃泌素仅使胃蛋白酶释放略有增加（每小时总含量的15%）。胃泌素的作用更显著，引起胃蛋白酶分泌显著增加，最高可达每小时总胃蛋白酶含量的28%。胃泌素的半数有效浓度（CD50）为0.061 nM。组胺和英普咪定同样使胃蛋白酶分泌显著增加（最高分别为每小时总储存量的40%和35%）。当将组胺的CD50（0.718 microM）与英普咪定的CD50（0.011 microM）进行比较时，按摩尔计算，英普咪定作为胃蛋白酶促分泌剂的效力比组胺高65倍。当比较胃泌素、组胺和英普咪定刺激期间的胃蛋白酶和酸反应时，胃蛋白酶原分泌向左偏移，因此比酸分泌对刺激更敏感。
