Caglar Yusuf Sukru, Demirel Altan, Dogan Ihsan, Huseynov Ramis, Eroglu Umit, Ozgural Onur, Cansiz Cevriye, Bahadir Burak, Kilinc Mustafa Cemil, Al-Beyati Eyyub S M
Department of Neurosurgery, Ankara University, Faculty of Medicine, Ankara, Turkey.
Department of Neurosurgery, Aksaray University, Aksaray Training and Research Hospital, Aksaray, Turkey.
World Neurosurg. 2018 Jun;114:e247-e253. doi: 10.1016/j.wneu.2018.02.171. Epub 2018 Mar 10.
The pathophysiology of spinal cord injury (SCI) with the information obtained to date has not been elucidated fully. A safe drug or treatment protocol that results in cell regeneration for SCI remains unknown. Neuroprotective and neuroregenerative effects of riluzole, administered after a SCI, have been shown in experimental studies. This study aimed to investigate the effect of riluzole on neural regeneration in a rat SCI model.
Thirty-two rats were divided into 8 groups, with 4 rats in each group. Hemisection method was performed after T7-T9 laminectomy. Rats were intraperitoneally aministered with riluzole (6 mg/kg). Locomotor recovery of the rats was assessed at 1 day, and 1, 2, 3, and 4 weeks after the 21-point Basso, Beattie, and Bresnahan test. Subsequently, the spinal cords of the rats were scored according to a semiquantitative grading system using a light microscope, and the numbers of myelinated axons, neurons, and glial cells were calculated.
Basso, Beattie, and Bresnahan test changes were statistically significant when groups 4-6 and 8 were compared with the other groups (P < 0.05, P < 0.00625). The results of the numbers of neurons, glial cells, and myelinated axons were statistically significant. Especially group 8, in which riluzole was administered 5 days before injury, more positive clinical and histopathologic results were obtained.
Riluzole treatment is more effective when provided before injury. Riluzole may contribute to functional recovery when used in the preoperative period in patients who are at a high risk for permanent neurologic deficit.
迄今为止，利用已获取的信息尚未完全阐明脊髓损伤（SCI）的病理生理学。一种能实现SCI细胞再生的安全药物或治疗方案仍然未知。实验研究表明，在脊髓损伤后给予利鲁唑具有神经保护和神经再生作用。本研究旨在探讨利鲁唑对大鼠SCI模型神经再生的影响。
32只大鼠分为8组，每组4只。在T7 - T9椎板切除术后采用半横断法。大鼠腹腔注射利鲁唑（6 mg/kg）。在术后1天以及1、2、3和4周，通过21分的Basso、Beattie和Bresnahan测试评估大鼠的运动恢复情况。随后，使用光学显微镜根据半定量分级系统对大鼠脊髓进行评分，并计算有髓轴突、神经元和神经胶质细胞的数量。
将第4 - 6组和第8组与其他组进行比较时，Basso、Beattie和Bresnahan测试变化具有统计学意义（P < 0.05，P < 0.00625）。神经元、神经胶质细胞和有髓轴突数量的结果具有统计学意义。特别是第8组，在损伤前5天给予利鲁唑，获得了更积极的临床和组织病理学结果。
在损伤前给予利鲁唑治疗更有效。对于有永久性神经功能缺损高风险的患者，术前使用利鲁唑可能有助于功能恢复。
