通道视紫红质-2的过表达会干扰GABAb受体介导的前额叶皮质含生长抑素中间神经元释放γ-氨基丁酸的抑制作用。
Overexpression of channelrhodopsin-2 interferes with the GABAb receptor-mediated depression of GABA release from the somatostatin-containing interneurons of the prefrontal cortex.
作者信息
Liu Lei, Ito Wataru, Morozov Alexei
机构信息
Virginia Tech Carilion Research Institute, Roanoke, Virginia, United States.
Virginia Tech, School of Biomedical Engineering and Sciences, Blacksburg, Virginia, United States.
出版信息
Neurophotonics. 2018 Apr;5(2):025003. doi: 10.1117/1.NPh.5.2.025003. Epub 2018 Mar 1.
Abstract
Region and cell-type restricted expression of light-activated ion channels is the indispensable tool to study properties of synapses in specific circuits and to monitor synaptic alterations by various stimuli including neuromodulators and behaviors, both and . These analyses require the light-activated proteins or viral vectors for their delivery that do not interfere with the phenomenon under study. Here, we report a case of such interference in which the high-level expression of channelrhodopsin-2 introduced in the somatostatin-positive GABAergic neurons of the dorsomedial prefrontal cortex by an adeno-associated virus vector weakens the presynaptic GABAb receptor-mediated suppression of GABA release.
摘要
光激活离子通道的区域和细胞类型限制性表达是研究特定神经回路中突触特性以及监测包括神经调质和行为在内的各种刺激引起的突触变化所不可或缺的工具。这些分析需要光激活蛋白或用于递送的病毒载体，且它们不会干扰所研究的现象。在此，我们报告了这样一种干扰情况：腺相关病毒载体将2型视紫红质通道蛋白引入背内侧前额叶皮质生长抑素阳性GABA能神经元中进行高水平表达，会削弱突触前GABAB受体介导的GABA释放抑制作用。
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