Della-Colleta Heloisa H M, Carvalho Hernandes F
Department of Structural and Functional Biology, Institute of Biology, State University of Campinas - UNICAMP, Campinas, São Paulo, Brazil.
Biochem Cell Biol. 2018 Oct;96(5):556-563. doi: 10.1139/bcb-2017-0242. Epub 2018 Mar 13.
Hyaluronan (HA) has been implicated in tissue remodeling, healing, and tumor growth. This study investigated the variation in hyaluronan content, distribution, and metabolism in the rat ventral prostate (VP) in response to androgen deprivation after castration. The mRNA abundance of hyaluronan synthases (Has1-3) and hyaluronidases (Hyal 1-3) were assessed by reverse transcription (RT)-PCR and immunohistochemistry, respectively. The results demonstrated an increased concentration, but an overall reduction in HA content. HA was located in both epithelium and stroma of the prostate of both the noncastrated and castrated animals. Quantitative RT-PCR (qRT-PCR) showed that Has1 and Has2 are major synthases, and that Hyal 1 was the predominant hydrolase expressed in the VP. qRT-PCR also showed that Has1 and Has2 mRNA increased transiently after castration, whereas Has3 mRNA declined markedly. While Hyal 1 mRNA increased slowly up to day 21 after castration, Hyal 2 and Hyal 3 mRNA dropped significantly. CD44 was found in the epithelial cells and in some stromal cells in both hormonal conditions. In conclusion, castration results in increased abundance of Has1 and Has2 mRNA, but is associated with a decrease in the total content of HA, with an increased concentration, and a predominance of short-chain HA molecules.
透明质酸（HA）与组织重塑、愈合及肿瘤生长有关。本研究调查了去势后雄激素剥夺对大鼠腹侧前列腺（VP）中透明质酸含量、分布及代谢的影响。分别通过逆转录（RT）-PCR和免疫组化评估透明质酸合酶（Has1-3）和透明质酸酶（Hyal 1-3）的mRNA丰度。结果显示浓度增加，但HA含量总体减少。HA位于未去势和去势动物前列腺的上皮和基质中。定量RT-PCR（qRT-PCR）表明Has1和Has2是主要合酶，Hyal 1是VP中表达的主要水解酶。qRT-PCR还显示去势后Has1和Has2 mRNA短暂增加，而Has3 mRNA显著下降。去势后直至第21天，Hyal 1 mRNA缓慢增加，而Hyal 2和Hyal 3 mRNA显著下降。在两种激素状态下，上皮细胞和一些基质细胞中均发现了CD44。总之，去势导致Has1和Has2 mRNA丰度增加，但与HA总含量减少、浓度增加以及短链HA分子占优势有关。
