College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China; State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, Donghua University, Shanghai 201620, China.
College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China; State Environmental Protection Engineering Center for Pollution Treatment and Control in Textile Industry, Donghua University, Shanghai 201620, China; School of Environment and Surveying Engineering, Suzhou University, Anhui 234000, China.
Chemosphere. 2018 Jun;201:578-585. doi: 10.1016/j.chemosphere.2018.03.025. Epub 2018 Mar 5.
In present study, a hydrolysis acidification (HA) reactor was used for simulated dyeing wastewater treatment. Co-substrates included starch, glucose, sucrose, yeast extract (YE) and peptone were fed sequentially into the HA reactor to enhance the HA process effects. The performance of the HA reactor and the microbial community structure in HA process were investigated under different co-substrates conditions. Results showed that different co-substrates had different influences on the performance of HA reactor. The highest decolorization (50.64%) and COD removal rate (60.73%) of the HA reactor were obtained when sucrose was as the co-substrate. And it found that carbon co-substrates starch, glucose and sucrose exhibited better decolorization and higher COD removal efficiency of the HA reactor than the nitrogen co-substrates YE and peptone. Microbial community structure in the HA process was analyzed by Illumina MiSeq sequencing. Results revealed different co-substrates had different influences on the community structure and microbial diversity in HA process. It was considered that sucrose could enrich the species such as Raoultella, Desulfovibrio, Tolumonas, Clostridium, which might be capable of degrading the dyes. Sucrose was considered to be the best co-substrate of enhancing the HA reactor's performance in this study. This work would provide deep insight into the influence of many different co-substrates on HA reactor performance and microbial communities in HA process.
在本研究中，采用水解酸化（HA）反应器处理模拟染料废水。连续向 HA 反应器中添加淀粉、葡萄糖、蔗糖、酵母提取物（YE）和蛋白胨作为共基质，以增强 HA 工艺效果。在不同共基质条件下，考察了 HA 反应器的性能和 HA 工艺中的微生物群落结构。结果表明，不同共基质对 HA 反应器的性能有不同的影响。当蔗糖作为共基质时，HA 反应器的脱色率（50.64%）和 COD 去除率（60.73%）最高。并且发现碳共基质淀粉、葡萄糖和蔗糖比氮共基质 YE 和蛋白胨对 HA 反应器的脱色和 COD 去除效率更高。通过 Illumina MiSeq 测序分析了 HA 工艺中的微生物群落结构。结果表明，不同共基质对 HA 工艺中的群落结构和微生物多样性有不同的影响。认为蔗糖可以富集如 Raoultella、Desulfovibrio、Tolumonas、Clostridium 等物种，这些物种可能能够降解染料。蔗糖被认为是本研究中增强 HA 反应器性能的最佳共基质。这项工作将深入了解许多不同共基质对 HA 反应器性能和 HA 工艺中微生物群落的影响。
