Evershed R P, Goad L J
Biomed Environ Mass Spectrom. 1987 Mar;14(3):131-40. doi: 10.1002/bms.1200140307.
Mixtures of both synthetic and naturally occurring (human plasma cholesteryl esters have been examined by capillary gas chromatography/mass spectrometry (GC/MS). A magnetic sector mass spectrometer was used and a variety of ionization modes were assessed with a view to obtaining structural information on intact cholesteryl esters. By employing ammonia as reagent gas, with negative ion scanning, spectra were produced from which the nature of steryl and fatty acyl moieties could be readily deduced. Analyses were performed at an ion source temperature of 300 degrees C in order to maintain the integrity of the gas chromatographic profile. The technique described is of general use for the GC/MS analysis of steryl esters, particularly in conjunction with magnetic sector instruments.
已通过毛细管气相色谱/质谱联用仪（GC/MS）对合成及天然存在的（人血浆胆固醇酯）混合物进行了检测。使用了磁扇型质谱仪，并评估了多种电离模式，以期获得完整胆固醇酯的结构信息。通过使用氨气作为反应气并进行负离子扫描，生成了能轻松推断出甾醇和脂肪酰基部分性质的光谱。为保持气相色谱图的完整性，分析在300摄氏度的离子源温度下进行。所描述的技术普遍适用于甾醇酯的GC/MS分析，特别是与磁扇型仪器结合使用时。
