Orikasa Yoshitake, Oda Yuji, Ohwada Takuji
Department of Life and Food Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.
Microorganisms. 2018 Mar 13;6(1):26. doi: 10.3390/microorganisms6010026.
NBRC 32995 was found to hydrolyze fructooligosaccharides (FOS), as well as sucrose, almost completely into monosaccharides through the production of sufficient amounts of organic acids, indicating that the complete hydrolysis of FOS was caused by the secretion of β-fructofuranosidase from fungal cells. Thus, the gene, encoding a β-fructofuranosidase, was amplified by degenerate PCR, and its complete nucleotide sequence was determined. The total length of the gene was 1590 bp, and the SucA protein of NBRC 32995 belonged to clade VIa, which also contains and is closely related to Saccharomycotina, a subdivision of the Ascomycota.
发现NBRC 32995能够水解低聚果糖（FOS）以及蔗糖，通过产生足够量的有机酸几乎将它们完全转化为单糖，这表明FOS的完全水解是由真菌细胞分泌的β-呋喃果糖苷酶引起的。因此，通过简并PCR扩增了编码β-呋喃果糖苷酶的基因，并确定了其完整的核苷酸序列。该基因的全长为1590 bp，NBRC 32995的SucA蛋白属于第六类，该类还包含子囊菌门的一个亚门酵母亚门，并且与之密切相关。
