Shiokawa K, Tashiro K, Yamana K, Sameshima M
Cell Differ. 1987 Apr;20(4):253-61. doi: 10.1016/0045-6039(87)90470-2.
When bacteriophage lambda DNA was injected into the cytoplasm of the fertilized egg of Xenopus laevis, giant nucleus-like structures were assembled around the injected DNA. These nucleus-like structures survived during cleavage and were partitioned into blastomeres at the blastula stage. The nucleus-like structures formed in the uncleaved fertilized eggs and the blastula cells were both surrounded by a bilayer nuclear membrane with nuclear pore complexes. The ultrastructural features of the lambda DNA-induced nucleus-like structure were considerably different from those of the normal blastula nucleus: although the nuclear pore complexes appeared to be normal, the 'nucleoplasm' was much too homogeneous as compared with that of the normal nucleus.
当将噬菌体λDNA注入非洲爪蟾受精卵的细胞质中时，在注入的DNA周围会组装形成巨大的核样结构。这些核样结构在卵裂过程中存活下来，并在囊胚期被分配到卵裂球中。在未分裂的受精卵和囊胚细胞中形成的核样结构都被带有核孔复合体的双层核膜所包围。λDNA诱导形成的核样结构的超微结构特征与正常囊胚核的超微结构特征有很大不同：尽管核孔复合体看起来是正常的，但与正常细胞核相比，其“核质”过于均匀。
