Hofmann A, Montag M, Steinbeisser H, Trendelenburg M F
Institute of Experimental Pathology, German Cancer Research Center, Heidelberg.
Cell Differ Dev. 1990 Apr;30(1):77-85. doi: 10.1016/0922-3371(90)90075-8.
The fate of DNA injected into in vitro fertilized eggs of Xenopus laevis during subsequent early embryogenesis was investigated by changing the time period and the area of injection. Form I/II plasmid DNA was found to be preferentially replicated in embryos which had been injected 60-65 min after fertilization into the animal half of the fertilized egg, irrespective of the presence of a eukaryotic origin of replication sequence element in the DNA probe used for injection. In the experiments where plasmid DNA and lambda-DNA were coinjected, only the latter was actively replicated, which suggests an inhibitory activity of this DNA on replication of coinjected plasmid DNA.
