Bitoh S, Yamamoto H, Fujimoto S
Cell Immunol. 1987 Jun;107(1):148-57. doi: 10.1016/0008-8749(87)90275-9.
Mouse B-cell line, established by culturing anti-Thy-1 and complement-treated splenic B cells with concanavalin A-stimulated conditioned medium, expressed immunoglobulins and Ia antigens on its surface. The long-term-cultured B-cell line was split in two and maintained with or without 3300 R X-irradiated T-cell-depleted syngeneic splenic adherent cells (SAC). Interestingly, the B-cell line cultured without SAC lost its Ia antigen but not its Ig expression, whereas the cell line with SAC maintained both Ia and Ig expression. The ability to express Ia antigens was restored by culturing them only in the presence of Ia-positive feeder cells. Neither recombinant interferon-gamma or lectin-stimulated conditioned medium nor cell-free culture supernatant SAC had the ability to restore Ia antigen expression on the B-cell line. Incubation of Ia-negative B-cell line with phorbol esters restored the Ia expression. It is suggested that the expression of Ia antigen on B lymphocytes was controlled differently from that on macrophage lineage. The B-cell line expressing Ia antigens acts as stimulator cells for alloantigen-activated T lymphocytes and as antigen-presenting cells on the KLH-specific Ia-restricted proliferative T-cell clone in the presence of a specific antigen.
通过用伴刀豆球蛋白A刺激的条件培养基培养抗Thy-1和经补体处理的脾B细胞建立的小鼠B细胞系，在其表面表达免疫球蛋白和Ia抗原。将长期培养的B细胞系一分为二，并在有或没有经3300伦琴X射线照射的T细胞去除的同基因脾黏附细胞（SAC）的情况下进行维持培养。有趣的是，在没有SAC的情况下培养的B细胞系失去了其Ia抗原，但保留了Ig表达，而有SAC的细胞系则同时维持了Ia和Ig表达。仅在Ia阳性饲养细胞存在的情况下培养它们，可恢复表达Ia抗原的能力。重组干扰素-γ或凝集素刺激的条件培养基以及无细胞培养上清液SAC均无能力恢复B细胞系上Ia抗原的表达。用佛波酯孵育Ia阴性B细胞系可恢复Ia表达。提示B淋巴细胞上Ia抗原的表达调控方式与巨噬细胞系不同。表达Ia抗原的B细胞系在存在特异性抗原的情况下，作为同种异体抗原激活的T淋巴细胞的刺激细胞以及针对钥孔戚血蓝蛋白特异性Ia限制的增殖性T细胞克隆的抗原呈递细胞。
