Savinova O S, Solyev P N, Vasina D V, Tyazhelova T V, Fedorova T V, Savinova T S
Bach Institute of Biochemistry, Biotechnology Research Center, Russian Academy of Sciences, Moscow, 119071, Russia.
Biochemistry (Mosc). 2018 Jan;83(1):26-31. doi: 10.1134/S0006297918010030.
The ability of the ascomycete Aspergillus niger N402 to transform exogenous progesterone was investigated. We found that this strain has steroid-hydroxylating activity and can introduce a hydroxyl group into the progesterone molecule mainly at positions C11(α) and C21 with predominant formation of 21-hydroxyprogesterone (deoxycortone). In addition, formation of 6β,11α-dihydroxyprogesterone was also observed. Studying the effects of the growth medium composition and temperature on progesterone conversion by A. niger N402 showed that the most intense accumulation of 21-hydroxyprogesterone occurred in minimal synthetic medium at 28°C. Increasing the cultivation temperature to 37°C resulted in almost complete inhibition of the hydroxylase activity in the minimal medium. In the complete medium, a similar increase in temperature inhibited 11α-hydroxylase activity and completely suppressed 6β-hydroxylase activity, but it produced no effect on 21-hydroxylating activity.
研究了子囊菌黑曲霉N402转化外源性孕酮的能力。我们发现该菌株具有类固醇羟化活性，能够主要在C11（α）和C21位将羟基引入孕酮分子，主要生成21-羟孕酮（脱氧皮质酮）。此外，还观察到6β，11α-二羟孕酮的形成。研究生长培养基组成和温度对黑曲霉N402转化孕酮的影响表明，21-羟孕酮的最强烈积累发生在28°C的基本合成培养基中。将培养温度提高到37°C导致基本培养基中的羟化酶活性几乎完全受到抑制。在完全培养基中，类似的温度升高抑制了11α-羟化酶活性并完全抑制了6β-羟化酶活性，但对21-羟化活性没有影响。
