Department of Cell Biology, University of Alberta, Edmonton, Alberta, Canada, T6G 2H7.
Laboratory of Molecular and Evolutionary Parasitology, RAPID group, School of Biosciences, University of Kent, Canterbury, Kent CT2 7NJ, UK.
J Cell Sci. 2018 Apr 9;131(7):jcs213306. doi: 10.1242/jcs.213306.
Although the Golgi complex has a conserved morphology of flattened stacked cisternae in most eukaryotes, it has lost the stacked organisation in several lineages, raising the question of what range of morphologies is possible for the Golgi. In order to understand this diversity, it is necessary to characterise the Golgi in many different lineages. Here, we identify the Golgi complex in , one of the first descriptions of an unstacked Golgi organelle in a non-parasitic eukaryote, other than fungi. We provide a comprehensive list of Golgi-associated membrane trafficking genes encoded in two species of and show that nearly all are expressed in mouse-passaged cells. We then study distribution of the Golgi marker ()CopB by fluorescence in , identifying membranous structures that are disrupted by Brefeldin A treatment, consistent with Golgi localisation. Confocal and immunoelectron microscopy reveals that COPB localises to tubular membranous structures. Our data identify the Golgi organelle for the first time in this major eukaryotic lineage, and provide the rare example of a tubular morphology, representing an important sampling point for the comparative understanding of Golgi organellar diversity.This article has an associated First Person interview with the first author of the paper.
尽管高尔基体在大多数真核生物中具有扁平堆叠的潴泡的保守形态，但在几个谱系中它已经失去了堆叠的组织，这就提出了高尔基体可能具有的形态范围的问题。为了理解这种多样性，有必要在许多不同的谱系中对高尔基体进行特征描述。在这里，我们鉴定了 中的高尔基体复合物，这是除真菌以外的第一个非寄生真核生物中非堆叠高尔基体细胞器的描述之一。我们提供了两种 物种中编码的与高尔基体相关的膜运输基因的综合列表，并表明几乎所有基因在经过小鼠传代的 细胞中都有表达。然后，我们通过荧光研究了 中高尔基体标记物（）CopB 的分布，鉴定出布雷菲德菌素 A 处理破坏的膜结构，这与高尔基体定位一致。共聚焦和免疫电子显微镜显示 COPB 定位于管状膜结构上。我们的数据首次在这个主要的真核生物谱系中鉴定出了高尔基体细胞器，并提供了管状形态的罕见示例，代表了对高尔基体细胞器多样性进行比较理解的重要采样点。本文有该论文第一作者的相关第一人称采访。
