Department of Biological Sciences, Rutgers University, Newark, NJ 07102, USA.
J Eukaryot Microbiol. 2013 Mar-Apr;60(2):166-78. doi: 10.1111/jeu.12019. Epub 2013 Jan 14.
This study provides evidence for the Golgi-like activity of the multilayered interlaced network (MIN) and new ultrastructural observations of the MIN in the sporoplasm of Anncaliia algerae, a microsporidium that infects both insects and humans. The MIN is attached to the end of the polar tubule upon extrusion from the germinating spore. It surrounds the sporoplasm, immediately below its plasma membrane, and most likely maintains the integrity of the sporoplasm, as it is pulled through the everting polar tube. Furthermore, the MIN appears to deposit its dense contents on the surface of the sporoplasm within minutes of spore discharge thickening the plasma membrane. This thickening is characteristic of the developmental stages of the genus Anncaliia. The current study utilizes transmission electron microscopy (TEM), enzyme histochemistry, and high voltage TEM (HVEM) with 3D tomographic reconstruction to both visualize the structure of the MIN and demonstrate that the MIN is a Golgi-related structure. The presence of developmentally regulated Golgi in the Microsporidia has been previously documented. The current study extends our understanding of the microsporidial Golgi and is consistent with the MIN being involved in the extracellular secretion in Anncaliia algerae. This report further illustrates the unique morphology of the MIN as illustrated by HVEM using 3D tomography.
本研究为多层交织网络（MIN）的高尔基样活性提供了证据，并对感染昆虫和人类的微孢子虫 Anncaliia algerae 中 MIN 的超微结构进行了新的观察。MIN 在从萌发孢子中挤出时附着在极管的末端。它环绕着孢子质，就在其质膜的下方，并且很可能在通过外翻的极管拉动时保持孢子质的完整性。此外，MIN 似乎在孢子排出后的几分钟内将其密集的内容物沉积在孢子质的表面上，使质膜变厚。这种增厚是 Anncaliia 属发育阶段的特征。本研究利用透射电子显微镜（TEM）、酶组织化学和高压 TEM（HVEM）与 3D 断层重建，既可视化了 MIN 的结构，又证明了 MIN 是一种与高尔基体相关的结构。微孢子虫中发育调节的高尔基体以前有过记录。本研究扩展了我们对微孢子虫高尔基体的理解，并且与 MIN 参与 Anncaliia algerae 的细胞外分泌一致。本报告进一步说明了 MIN 的独特形态，HVEM 使用 3D 断层重建进行了说明。
